| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-REC8 (194-561) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Rec8 |
| Gene ID: |
56739 |
| Uniprot ID: |
REC8_MOUSE |
| Immunogen Region: |
194-561 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 194-561 of mouse REC8 (NP_064386.2). |
| Immunogen Sequence: |
EVITLQEAEPIRMLQIEGEQ DLPEISRGDLELLIAEKDDA ILLEERQRGRLLRQRRASLP LDESREEPRALEGAGLVSAL SPPAPAQVEGIQEALPGQVF PPEVQKMTGWEPGALLTEVT PPQELRLPAPPSTEKRLPSL QRPLPRRHRRRQLLFWDKET QISREKFEEQLQTGAHCWEY PVAQPPKRMLTSPAELFRTP TLSGWLPPELLGLWTHCAQV PQRMLRQRPQLETEETVEE |
| Tissue Specificity | Expressed primarily in the gonads. In the testis, expressed in pachytene spermatocytes and in spermatids. Not expressed in spermatogonia or somatic cells. In the ovary, expressed only in oocytes. Low levels also detected in a number of somatic tissues including thymus, lung, liver, kidney and small intestine. |
| Post Translational Modifications | Phosphorylated. |
| Function | Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. |
| Protein Name | Meiotic Recombination Protein Rec8 HomologCohesin Rec8p |
| Cellular Localisation | NucleusChromosomeCentromereIn Meiotic ChromosomesLocalized Along Axial Elements In Prophase From The Leptotene To Diplotene StagesAt Later Prophase StagesDiakinesis And Metaphase ILocalized Along Interstitial Axes Of Chromosomes Including Both Centromere And Arm RegionsNo Longer Detected In Arm Regions In Anaphase I But Persists On Centromere Regions Until Metaphase Ii |
| Alternative Antibody Names | Anti-Meiotic Recombination Protein Rec8 Homolog antibodyAnti-Cohesin Rec8p antibodyAnti-Rec8 antibodyAnti-Mei8 antibodyAnti-Rec8L1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
