| Short Description : | Rabbit polyclonal REC8 (1-270) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | REC8 |
| Gene ID: | 9985 |
| Uniprot ID: | REC8_HUMAN |
| Immunogen Region: | 1-270 |
| Immunogen Sequence: | MFYYPNVLQRHTGCFATIWL AATRGSRLVKREYLRVNVVK TCEEILNYVLVRVQPPQPGL PRPRFSLYLSAQLQIGVIRV YSQQCQYLVEDIQHILERLH RAQLQIRIDMETELPSLLLP NHLAMMETLEDAPDPFFGMM SVDPRLPSPFDIPQIRHLLE AAIPERVEEIPPEVPTEPRE PERIPVTVLPPEAITILEAE PIRMLEIEGERELPEVSRRE LDLLIAEEEEAILLEIPRL |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human REC8 (NP_005123.2). |
| Tissue Specificity | Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa (at protein level). |
| Post Translational Modifications | Phosphorylated. |
| Function | Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. |
| Protein Name | Meiotic Recombination Protein Rec8 HomologCohesin Rec8p |
| Database Links | Reactome: R-HSA-1221632 |
| Cellular Localisation | NucleusChromosomeCentromereIn Meiotic ChromosomesLocalized Along Axial Elements In Prophase From The Leptotene To Diplotene StagesAt Later Prophase StagesDiakinesis And Metaphase ILocalized Along Interstitial Axes Of Chromosomes Including Both Centromere And Arm RegionsNo Longer Detected In Arm Regions In Anaphase I But Persists On Centromere Regions Until Metaphase IiLocalized To Centromeres And Spindle Poles In Endopolyploid Tumor Cells |
| Alternative Antibody Names | Anti-Meiotic Recombination Protein Rec8 Homolog antibodyAnti-Cohesin Rec8p antibodyAnti-REC8 antibodyAnti-REC8L1 antibody |
Information sourced from Uniprot.org
