• Immunofluorescence analysis of U-2 OS cells using RCN1 Rabbit polyclonal antibody (STJ11104431) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using RCN1 Rabbit polyclonal antibody (STJ11104431) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using RCN1 Rabbit polyclonal antibody (STJ11104431) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RCN1 antibody (30-331) (STJ11104431)

SKU:
STJ11104431

€140.63 - €655.63
Current Stock:
Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RCN1
Gene ID: 5954
Uniprot ID: RCN1_HUMAN
Immunogen Region: 30-331
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 30-331 of human RCN1 (NP_002892.1).
Immunogen Sequence: KPTVRKERVVRPDSELGERP PEDNQSFQYDHEAFLGKEDS KTFDQLTPDESKERLGKIVD RIDNDGDGFVTTEELKTWIK RVQKRYIFDNVAKVWKDYDR DKDDKISWEEYKQATYGYYL GNPAEFHDSSDHHTFKKMLP RDERRFKAADLNGDLTATRE EFTAFLHPEEFEHMKEIVVL ETLEDIDKNGDGFVDQDEYI ADMFSHEENGPEPDWVLSER EQFNEFRDLNKDGKLDKDE
Post Translational Modifications O-glycosylated. O-mannosylated by POMT1 and POMT2 and elongated by POMGNT1.
Function May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.
Protein Name Reticulocalbin-1
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Reticulocalbin-1 antibody
Anti-RCN1 antibody
Anti-RCN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance