| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RC3H1 (834-1133) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RC3H1 |
| Gene ID: |
149041 |
| Uniprot ID: |
RC3H1_HUMAN |
| Immunogen Region: |
834-1133 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 834-1133 of human RC3H1 (NP_742068.1). |
| Immunogen Sequence: |
AAGSVEMMNVESKGMRDQRL DLQRRAAETSDDDLIPFGDR PTVSRFGAISRTSKTIYQGA GPMQAMAPQGAPTKSINISD YSPYGTHGGWGASPYSPHQN IPSQGHFSERERISMSEVAS HGKPLPSAEREQLRLELQQL NHQISQQTQLRGLEAVSNRL VLQREANTLAGQSQPPPPPP PKWPGMISSEQLSLELHQVE REIGKRTRELSMENQCSLDM KSKLNTSKQAENGQPEPQN |
| Tissue Specificity | Widely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver. |
| Post Translational Modifications | Proteolytically cleaved after Arg-510 and Arg-579 by MALT1 in activated CD4(+) T cells.cleavage at Arg-510 and Arg-579 is critical for promoting RC3H1 degradation in response to T-cell receptor (TCR) stimulation, and hence is necessary for prolonging the stability of a set of mRNAs controlling Th17 cell differentiation. |
| Function | Post-transcriptional repressor of mRNAs containing a conserved stem loop motif, called constitutive decay element (CDE), which is often located in the 3'-UTR, as in HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF, TNFRSF4 and in many more mRNAs. Cleaves translationally inactive mRNAs harboring a stem-loop (SL), often located in their 3'-UTRs, during the early phase of inflammation in a helicase UPF1-independent manner. Binds to CDE and promotes mRNA deadenylation and degradation. This process does not involve miRNAs. In follicular helper T (Tfh) cells, represses of ICOS and TNFRSF4 expression, thus preventing spontaneous Tfh cell differentiation, germinal center B-cell differentiation in the absence of immunization and autoimmunity. In resting or LPS-stimulated macrophages, controls inflammation by suppressing TNF expression. Also recognizes CDE in its own mRNA and in that of paralogous RC3H2, possibly leading to feedback loop regulation. Recognizes and binds mRNAs containing a hexaloop stem-loop motif, called alternative decay element (ADE). Together with ZC3H12A, destabilizes TNFRSF4/OX40 mRNA by binding to the conserved stem loop structure in its 3'UTR. Able to interact with double-stranded RNA (dsRNA). miRNA-binding protein that regulates microRNA homeostasis. Enhances DICER-mediated processing of pre-MIR146a but reduces mature MIR146a levels through an increase of 3' end uridylation. Both inhibits ICOS mRNA expression and they may act together to exert the suppression. Acts as a ubiquitin E3 ligase. Pairs with E2 enzymes UBE2A, UBE2B, UBE2D2, UBE2F, UBE2G1, UBE2G2 and UBE2L3 and produces polyubiquitin chains. Shows the strongest activity when paired with UBE2N:UBE2V1 or UBE2N:UBE2V2 E2 complexes and generate both short and long polyubiquitin chains. |
| Protein Name | Roquin-1RoquinRing Finger And C3h Zinc Finger Protein 1Ring Finger And Ccch-Type Zinc Finger Domain-Containing Protein 1Ring Finger Protein 198 |
| Cellular Localisation | CytoplasmP-BodyCytoplasmic GranuleDuring StressSuch As That Induced By Arsenite TreatmentLocalizes To Cytosolic Stress GranulesLocalization To Stress GranulesBut Not To P-BodiesDepends Upon The Ring-Type Zinc FingerIcos Repression May Correlate With The Localization To P-BodiesNot To Stress Granules |
| Alternative Antibody Names | Anti-Roquin-1 antibodyAnti-Roquin antibodyAnti-Ring Finger And C3h Zinc Finger Protein 1 antibodyAnti-Ring Finger And Ccch-Type Zinc Finger Domain-Containing Protein 1 antibodyAnti-Ring Finger Protein 198 antibodyAnti-RC3H1 antibodyAnti-KIAA2025 antibodyAnti-RNF198 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
