| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase RBX1 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RBX1 |
| Gene ID: |
9978 |
| Uniprot ID: |
RBX1_HUMAN |
| Specificity: |
This antibody detects endogenous levels of RBX1 at Human, Mouse |
| Immunogen: |
Synthesized peptide derived from human RBX1 |
| Function | E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Recruits the E2 ubiquitin-conjugating enzyme CDC34 to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. As part of a multisubunit complex composed of elongin BC complex (ELOB and ELOC), elongin A/ELOA, RBX1 and CUL5.polyubiquitinates monoubiquitinated POLR2A. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rbx1E3 Ubiquitin-Protein Transferase Rbx1Protein ZypRing Finger Protein 75Ring-Box Protein 1Rbx1Regulator Of Cullins 1Roc1 Cleaved Into - E3 Ubiquitin-Protein Ligase Rbx1 - N-Terminally ProcessedE3 Ubiquitin-Protein Transferase Rbx1 - N-Terminally Processed |
| Database Links | Reactome: R-HSA-110314Reactome: R-HSA-1170546Reactome: R-HSA-1234176Reactome: R-HSA-180585Reactome: R-HSA-195253Reactome: R-HSA-2122947Reactome: R-HSA-2644606Reactome: R-HSA-2644607Reactome: R-HSA-2894862Reactome: R-HSA-4641258Reactome: R-HSA-5610780Reactome: R-HSA-5610783Reactome: R-HSA-5610785Reactome: R-HSA-5632684Reactome: R-HSA-5658442Reactome: R-HSA-5696394Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-68949Reactome: R-HSA-8854050Reactome: R-HSA-8939902Reactome: R-HSA-8951664Reactome: R-HSA-9010553Reactome: R-HSA-9020702Reactome: R-HSA-9604323Reactome: R-HSA-9679191Reactome: R-HSA-9708530Reactome: R-HSA-9725371Reactome: R-HSA-9755511Reactome: R-HSA-9762114Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rbx1 antibodyAnti-E3 Ubiquitin-Protein Transferase Rbx1 antibodyAnti-Protein Zyp antibodyAnti-Ring Finger Protein 75 antibodyAnti-Ring-Box Protein 1 antibodyAnti-Rbx1 antibodyAnti-Regulator Of Cullins 1 antibodyAnti-Roc1 Cleaved Into - E3 Ubiquitin-Protein Ligase Rbx1 - N-Terminally Processed antibodyAnti-E3 Ubiquitin-Protein Transferase Rbx1 - N-Terminally Processed antibodyAnti-RBX1 antibodyAnti-RNF75 antibodyAnti-ROC1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
