Anti-RBPMS antibody (110-219) (STJ117590)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RBPMS (110-219) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RBPMS |
| Gene ID | 11030 |
| Uniprot ID | RBPMS_HUMAN |
| Immunogen Region | 110-219 |
| Immunogen Sequence | LVGTPNPSTPLPNTVPQFIA REPYELTVPALYPSSPEVWA PYPLYPAELAPALPPPAFTY PASLHAQCFSPEAKPNTPVF CPLLQQIRFVSGNVFVTYQP TADQQRELPC |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 110-219 of human RBPMS (NP_001008712.1). |
Additional Info
| Tissue Specificity | Ubiquitously expressed, at various levels depending on the isoform and the tissue. Strongly expressed in the heart, prostate, small intestine, large intestine, and ovary.moderately expressed in the placenta, lung, liver, kidney, pancreas, and testis.and poorly expressed in the skeletal muscle, spleen, thymus and peripheral leukocytes. |
| Function | Isoform A: RNA binding protein that mediates the regulation of pre-mRNA alternative splicing (AS). Acts either as activator (FLNB, HSPG2, LIPA1, MYOCD, PTPRF and PPFIBP1) or repressor (TPM1, ACTN1, ITGA7, PIEZO1, LSM14B, MBNL1 and MBML2) of splicing events on specific pre-mRNA targets. Together with RNA binding proteins RBFOX2 and MBNL1/2, activates a splicing program associated with differentiated contractile vascular smooth muscle cells (SMC) by regulating AS of numerous pre-mRNA involved in actin cytoskeleton and focal adhesion machineries, suggesting a role in promoting a cell differentiated state. Binds to introns, exons and 3'-UTR associated with tandem CAC trinucleotide motifs separated by a variable spacer region, at a minimum as a dimer. The minimal length of RNA required for RBPMS-binding tandem CAC motifs is 15 nt, with spacing ranging from 1 to 9 nt. Can also bind to CA dinucleotide repeats. Mediates repression of TPM1 exon 3 by binding to CAC tandem repeats in the flanking intronic regions, followed by higher-order oligomerization and heterotypic interactions with other splicing regulators including MBNL1 and RBFOX2, which prevents assembly of ATP-dependent splicing complexes. Isoform C: Acts as a regulator of pre-mRNA alternative splicing (AS). Binds mRNA. Regulates AS of ACTN1, FLNB, although with lower efficiency than isoform A / RBPMSA. Acts as coactivator of SMAD transcriptional activity in a TGFB1-dependent manner, possibly through increased phosphorylation of SMAD2 and SMAD3 at the C-terminal SSXS regions and promotion of the nuclear accumulation of SMAD proteins. |
| Protein Name | Rna-Binding Protein With Multiple SplicingRbp-MsRbpmsHeart And Rrm Expressed SequenceHermes |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmStress GranuleP-BodyLocalized To Cytoplasmic Stress Granules After Oxidative StressTranslocates Into Cytoplasmic Stress Granules That Probably Corresponds To P-Bodies In Response To Oxidative Stress |
| Alternative Antibody Names | Anti-Rna-Binding Protein With Multiple Splicing antibodyAnti-Rbp-Ms antibodyAnti-Rbpms antibodyAnti-Heart And Rrm Expressed Sequence antibodyAnti-Hermes antibodyAnti-RBPMS antibodyAnti-HERMES antibody |
Information sourced from Uniprot.org