Anti-RBM25 antibody (614-843) (STJ117691)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RBM25 (614-843) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RBM25 |
| Gene ID | 58517 |
| Uniprot ID | RBM25_HUMAN |
| Immunogen Region | 614-843 |
| Immunogen Sequence | KPTLRPISSAPSVSSASGNA TPNTPGDESPCGIIIPHENS PDQQQPEEHRPKIGLSLKLG ASNSPGQPNSVKRKKLPVDS VFNKFEDEDSDDVPRKRKLV PLDYGEDDKNATKGTVNTEE KRKHIKSLIEKIPTAKPELF AYPLDWSIVDSILMERRIRP WINKKIIEYIGEEEATLVDF VCSKVMAHSSPQSILDDVAM VLDEEAEVFIVKMWRLLIYE TEAKKIGLVK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 614-843 of human RBM25 (NP_067062.1). |
Additional Info
| Post Translational Modifications | Sumoylated. |
| Function | RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure. |
| Protein Name | Rna-Binding Protein 25Arg/Glu/Asp-Rich Protein Of 120 KdaRed120Protein S164Rna-Binding Motif Protein 25Rna-Binding Region-Containing Protein 7 |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | Nucleus SpeckleCytoplasmColocalizes PredominantlyWith Sfrs2 And Luc7l3 Splicing FactorsIn Nuclear SpecklesCytoplasmic Localization Is Faint |
| Alternative Antibody Names | Anti-Rna-Binding Protein 25 antibodyAnti-Arg/Glu/Asp-Rich Protein Of 120 Kda antibodyAnti-Red120 antibodyAnti-Protein S164 antibodyAnti-Rna-Binding Motif Protein 25 antibodyAnti-Rna-Binding Region-Containing Protein 7 antibodyAnti-RBM25 antibodyAnti-RNPC7 antibody |
Information sourced from Uniprot.org