• Immunofluorescence analysis of U-2 OS cells using RBM25 Rabbit polyclonal antibody (STJ117691) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using RBM25 Rabbit polyclonal antibody (STJ117691) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-RBM25 antibody (614-843) (STJ117691)

SKU:
STJ117691

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RBM25 (614-843) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RBM25
Gene ID: 58517
Uniprot ID: RBM25_HUMAN
Immunogen Region: 614-843
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 614-843 of human RBM25 (NP_067062.1).
Immunogen Sequence: KPTLRPISSAPSVSSASGNA TPNTPGDESPCGIIIPHENS PDQQQPEEHRPKIGLSLKLG ASNSPGQPNSVKRKKLPVDS VFNKFEDEDSDDVPRKRKLV PLDYGEDDKNATKGTVNTEE KRKHIKSLIEKIPTAKPELF AYPLDWSIVDSILMERRIRP WINKKIIEYIGEEEATLVDF VCSKVMAHSSPQSILDDVAM VLDEEAEVFIVKMWRLLIYE TEAKKIGLVK
Post Translational Modifications Sumoylated.
Function RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure.
Protein Name Rna-Binding Protein 25
Arg/Glu/Asp-Rich Protein Of 120 Kda
Red120
Protein S164
Rna-Binding Motif Protein 25
Rna-Binding Region-Containing Protein 7
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus Speckle
Cytoplasm
Colocalizes Predominantly
With Sfrs2 And Luc7l3 Splicing Factors
In Nuclear Speckles
Cytoplasmic Localization Is Faint
Alternative Antibody Names Anti-Rna-Binding Protein 25 antibody
Anti-Arg/Glu/Asp-Rich Protein Of 120 Kda antibody
Anti-Red120 antibody
Anti-Protein S164 antibody
Anti-Rna-Binding Motif Protein 25 antibody
Anti-Rna-Binding Region-Containing Protein 7 antibody
Anti-RBM25 antibody
Anti-RNPC7 antibody

Information sourced from Uniprot.org

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