| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RASGRF1 |
| Gene ID: |
5923 |
| Uniprot ID: |
RGRF1_HUMAN |
| Immunogen Region: |
1-190 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RASGRF1 (NP_001139120.1). |
| Immunogen Sequence: |
MQKGIRLNDGHVASLGLLAR KDGTRKGYLSKRSSDNTKWQ TKWFALLQNLLFYFESDSSS RPSGLYLLEGCVCDRAPSPK PALSAKEPLEKQHYFTVNFS HENQKALELRTEDAKDCDEW VAAIAHASYRTLATEHEALM QKYLHLLQIVETEKTVAKQL RQQIEDGEIEIERLKAEITS LLKDNERIQS |
| Post Translational Modifications | Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome. Ubiquitinated and degraded following phosphorylation by PLK2. Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity. |
| Function | Promotes the exchange of Ras-bound GDP by GTP. |
| Protein Name | Ras-Specific Guanine Nucleotide-Releasing Factor 1Ras-Grf1Guanine Nucleotide-Releasing ProteinGnrpRas-Specific Nucleotide Exchange Factor Cdc25 |
| Database Links | Reactome: R-HSA-442982Reactome: R-HSA-5673001 |
| Alternative Antibody Names | Anti-Ras-Specific Guanine Nucleotide-Releasing Factor 1 antibodyAnti-Ras-Grf1 antibodyAnti-Guanine Nucleotide-Releasing Protein antibodyAnti-Gnrp antibodyAnti-Ras-Specific Nucleotide Exchange Factor Cdc25 antibodyAnti-RASGRF1 antibodyAnti-CDC25 antibodyAnti-GNRP antibodyAnti-GRF1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and MYOF knockout (KO) HeLa (KO) cells, using [KO Validated] MYOF Rabbit polyclonal antibody (STJ117622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/96581/379140/STJ117622_1__97810.1713146869.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MYOF knockout (KO) HeLa (KO) cells, using [KO Validated] MYOF Rabbit polyclonal antibody (STJ117622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")