| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Rap guanine nucleotide exchange factor 2 (190-270 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000ELISA 1:5000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RAPGEF2 |
| Gene ID: |
9693 |
| Uniprot ID: |
RPGF2_HUMAN |
| Immunogen Region: |
190-270 aa |
| Specificity: |
RPGF2 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: |
Synthesized peptide derived from the human protein at the amino acid range 190-270 |
| Post Translational Modifications | Ubiquitinated by NEDD4, leading to proteasomal degradation. Phosphorylation by PLK2 promotes its activity. |
| Function | Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP or not. Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP-induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain-derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions. |
| Protein Name | Rap Guanine Nucleotide Exchange Factor 2Cyclic Nucleotide Ras GefCnrasgefNeural Rap Guanine Nucleotide Exchange ProteinNrap GepPdz Domain-Containing Guanine Nucleotide Exchange Factor 1Pdz-Gef1Ra-Gef-1Ras/Rap1-Associating Gef-1 |
| Database Links | Reactome: R-HSA-5673001 |
| Cellular Localisation | CytoplasmPerinuclear RegionCell MembraneLate EndosomeCell JunctionAssociated With The Synaptic Plasma MembraneColocalizes With Adrb1 At The Plasma MembraneSynaptosomeEnriched In Synaptic Plasma Membrane And Neuronal Cell BodyColocalized With Ctnnb1 At Cell-Cell ContactsLocalized Diffusely In The Cytoplasm Before Neuronal Growth Factor (Ngf) StimulationRecruited To Late Endosomes After Ngf StimulationColocalized With The High Affinity Nerve Growth Factor Receptor Ntrk1 At Late EndosomesTranslocated To The Perinuclear Region In A Rap1a-Dependent MannerTranslocated To The Cell Membrane |
| Alternative Antibody Names | Anti-Rap Guanine Nucleotide Exchange Factor 2 antibodyAnti-Cyclic Nucleotide Ras Gef antibodyAnti-Cnrasgef antibodyAnti-Neural Rap Guanine Nucleotide Exchange Protein antibodyAnti-Nrap Gep antibodyAnti-Pdz Domain-Containing Guanine Nucleotide Exchange Factor 1 antibodyAnti-Pdz-Gef1 antibodyAnti-Ra-Gef-1 antibodyAnti-Ras/Rap1-Associating Gef-1 antibodyAnti-RAPGEF2 antibodyAnti-KIAA0313 antibodyAnti-NRAPGEP antibodyAnti-PDZGEF1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
