| Host: | Rabbit |
| Applications: | IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Rap guanine nucleotide exchange factor 1 (470-519 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC 1:100-1:300 ELISA 1:5000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RAPGEF1 |
| Gene ID: | 2889 |
| Uniprot ID: | RPGF1_HUMAN |
| Immunogen Region: | 470-519 aa |
| Specificity: | C3G Polyclonal Antibody detects endogenous levels of C3G protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human RAPGEF1 at the amino acid range 470-519 |
| Post Translational Modifications | Phosphorylation at Tyr-504 enhances activity as Rap guanine nucleotide exchange factor. |
| Function | Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth. |
| Protein Name | Rap Guanine Nucleotide Exchange Factor 1 Crk Sh3-Binding Gnrp Guanine Nucleotide-Releasing Factor 2 Protein C3g |
| Database Links | Reactome: R-HSA-170968 Reactome: R-HSA-186763 Reactome: R-HSA-8875555 Reactome: R-HSA-9013423 Reactome: R-HSA-9027284 Reactome: R-HSA-912631 |
| Cellular Localisation | Early Endosome |
| Alternative Antibody Names | Anti-Rap Guanine Nucleotide Exchange Factor 1 antibody Anti-Crk Sh3-Binding Gnrp antibody Anti-Guanine Nucleotide-Releasing Factor 2 antibody Anti-Protein C3g antibody Anti-RAPGEF1 antibody Anti-GRF2 antibody |
Information sourced from Uniprot.org