• Western blot analysis of extracts of various cell lines, using RAP1A + RAP1B rabbit monoclonal antibody (STJ11102534) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using RAP1A + RAP1B Rabbit monoclonal antibody (STJ11102534) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using RAP1A + RAP1B rabbit monoclonal antibody (STJ11102534) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using RAP1A + RAP1B Rabbit monoclonal antibody (STJ11102534) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using RAP1A + RAP1B rabbit monoclonal antibody (STJ11102534) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using RAP1A + RAP1B Rabbit monoclonal antibody (STJ11102534) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using RAP1A + RAP1B rabbit monoclonal antibody (STJ11102534) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using RAP1A + RAP1B Rabbit monoclonal antibody (STJ11102534) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RAP1A/RAP1B antibody (70-150) [S4MR] (STJ11102534)

SKU:
STJ11102534

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAP1B
RAP1A
Gene ID: 5908
5906
Uniprot ID: RAP1B_HUMAN
RAP1A_HUMAN
Immunogen Region: 70-150
Specificity: A synthetic peptide corresponding to a sequence within amino acids 70-150 of human RAP1A + RAP1B (P61224).
Immunogen Sequence: LYMKNGQGFALVYSITAQST FNDLQDLREQILRVKDTDDV PMILVGNKCDLEDERVVGKE QGQNLARQWNNCAFLESSAK S
Tissue Specificity
Alternative Antibody Names Anti-Ras-Related Protein Rap-1b antibody
Anti-Gtp-Binding Protein Smg P21b antibody
Anti-RAP1B antibody
Anti-OK antibody
Anti-SW-cl.11 antibody
Anti-Ras-Related Protein Rap-1a antibody
Anti-C21kg antibody
Anti-G-22k antibody
Anti-Gtp-Binding Protein Smg P21a antibody
Anti-Ras-Related Protein Krev-1 antibody
Anti-RAP1A antibody
Anti-KREV1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance