![Western blot analysis of lysates from wild type (WT) and RAG2 knockout (KO) 293T cells, using [KO Validated] RAG2 Rabbit polyclonal antibody (STJ27593) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101228/388717/STJ27593_1__84376.1713158254.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and RAG2 knockout (KO) 293T cells, using [KO Validated] RAG2 Rabbit polyclonal antibody (STJ27593) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of various lysates using [KO Validated] RAG2 Rabbit polyclonal antibody (STJ27593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101228/388718/STJ27593_2__48197.1713158255.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] RAG2 Rabbit polyclonal antibody (STJ27593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western blot analysis of lysates from wild type (WT) and RAG2 knockout (KO) 293T cells, using [KO Validated] RAG2 Rabbit polyclonal antibody (STJ27593) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/101228/388717/STJ27593_1__84376.1713158254.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and RAG2 knockout (KO) 293T cells, using [KO Validated] RAG2 Rabbit polyclonal antibody (STJ27593) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-RAG2 antibody (351-527) (STJ27593)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RAG2 (351-527) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAG2 |
| Gene ID | 5897 |
| Uniprot ID | RAG2_HUMAN |
| Immunogen Region | 351-527 |
| Immunogen Sequence | AEDDTNEEQTTFTNSQTSTE DPGDSTPFEDSEEFCFSAEA NSFDGDDEFDTYNEDDEEDE SETGYWITCCPTCDVDINTW VPFYSTELNKPAMIYCSHGD GHWVHAQCMDLAERTLIHLS AGSNKYYCNEHVEIARALHT PQRVLPLKKPPMKSLRKKGS GKILTPAKKSFLRRLFD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 351-527 of human RAG2 (NP_000527.2). |
Additional Info
| Tissue Specificity | Cells of the B- and T-lymphocyte lineages. |
| Function | Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3. |
| Protein Name | V(Dj Recombination-Activating Protein 2Rag-2 |
| Database Links | Reactome: R-HSA-1266695Reactome: R-HSA-5687128 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-V(Dj Recombination-Activating Protein 2 antibodyAnti-Rag-2 antibodyAnti-RAG2 antibody |
Information sourced from Uniprot.org