• Western blot analysis using RAG-2 monoclonal antibody against RAG2-hIgGFc transfected HEK293 (1) cell lysate.

Anti-RAG2 antibody (350-527aa aa) [4D5] (STJ98352)

SKU:
STJ98352

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-VDJ recombination-activating protein 2 (350-527aa aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Monoclonal
Clone ID: 4D5
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAG2
Gene ID: 5897
Uniprot ID: RAG2_HUMAN
Immunogen Region: 350-527aa aa
Specificity: RAG-2 Monoclonal Antibody detects endogenous levels of RAG-2 protein.
Immunogen: Purified recombinant fragment of human RAG-2 (350-527aa) expressed in E. Coli.
Function Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3.
Protein Name V(Dj Recombination-Activating Protein 2
Rag-2
Database Links Reactome: R-HSA-1266695
Reactome: R-HSA-5687128
Cellular Localisation Nucleus
Alternative Antibody Names Anti-V(Dj Recombination-Activating Protein 2 antibody
Anti-Rag-2 antibody
Anti-RAG2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance