| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RAD54L (648-747) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RAD54L |
| Gene ID: |
8438 |
| Uniprot ID: |
RAD54_HUMAN |
| Immunogen Region: |
648-747 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 648-747 of human RAD54L (Q92698). |
| Immunogen Sequence: |
EEQDVERHFSLGELKELFIL DEASLSDTHDRLHCRRCVNS RQIRPPPDGSDCTSDLAGWN HCTDKWGLRDEVLQAAWDAA STAITFVFHQRSHEEQRGLR |
| Post Translational Modifications | Acetylated. Acetylation promotes interaction with BRD9, and subsequently with RAD54, which is essential for homologous recombination (HR). Phosphorylated. Phosphorylation at Ser-572 by NEK1 specifically in G2 phase allows efficient removal of RAD51 filaments from DNA. |
| Function | Plays an essential role in homologous recombination (HR) which is a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA (ssDNA) gaps, and stalled or collapsed replication forks. Acts as a molecular motor during the homology search and guides RAD51 ssDNA along a donor dsDNA thereby changing the homology search from the diffusion-based mechanism to a motor-guided mechanism. Also plays an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized. Once DNA strand exchange occured, dissociates RAD51 from nucleoprotein filaments formed on dsDNA. |
| Protein Name | Dna Repair And Recombination Protein Rad54-LikeRad54 HomologHhr54Hrad54 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna Repair And Recombination Protein Rad54-Like antibodyAnti-Rad54 Homolog antibodyAnti-Hhr54 antibodyAnti-Hrad54 antibodyAnti-RAD54L antibodyAnti-RAD54A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
