Anti-RAD54L antibody (648-747) (STJ11103212)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RAD54L (648-747) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAD54L |
| Gene ID | 8438 |
| Uniprot ID | RAD54_HUMAN |
| Immunogen Region | 648-747 |
| Immunogen Sequence | EEQDVERHFSLGELKELFIL DEASLSDTHDRLHCRRCVNS RQIRPPPDGSDCTSDLAGWN HCTDKWGLRDEVLQAAWDAA STAITFVFHQRSHEEQRGLR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 648-747 of human RAD54L (Q92698). |
Additional Info
| Post Translational Modifications | Acetylated. Acetylation promotes interaction with BRD9, and subsequently with RAD54, which is essential for homologous recombination (HR). Phosphorylated. Phosphorylation at Ser-572 by NEK1 specifically in G2 phase allows efficient removal of RAD51 filaments from DNA. |
| Function | Plays an essential role in homologous recombination (HR) which is a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA (ssDNA) gaps, and stalled or collapsed replication forks. Acts as a molecular motor during the homology search and guides RAD51 ssDNA along a donor dsDNA thereby changing the homology search from the diffusion-based mechanism to a motor-guided mechanism. Also plays an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized. Once DNA strand exchange occured, dissociates RAD51 from nucleoprotein filaments formed on dsDNA. |
| Protein Name | Dna Repair And Recombination Protein Rad54-LikeRad54 HomologHhr54Hrad54 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna Repair And Recombination Protein Rad54-Like antibodyAnti-Rad54 Homolog antibodyAnti-Hhr54 antibodyAnti-Hrad54 antibodyAnti-RAD54L antibodyAnti-RAD54A antibody |
Information sourced from Uniprot.org