| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-DNA repair and recombination protein RAD54-like (221-270 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RAD54L |
| Gene ID: | 8438 |
| Uniprot ID: | RAD54_HUMAN |
| Immunogen Region: | 221-270 aa |
| Specificity: | Rad54 Polyclonal Antibody detects endogenous levels of Rad54 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human RAD54L at the amino acid range 221-270 |
| Function | Plays an essential role in homologous recombination (HR) which is a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA (ssDNA) gaps, and stalled or collapsed replication forks. Acts as a molecular motor during the homology search and guides RAD51 ssDNA along a donor dsDNA thereby changing the homology search from the diffusion-based mechanism to a motor-guided mechanism. Also plays an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized. Once DNA strand exchange occured, dissociates RAD51 from nucleoprotein filaments formed on dsDNA. |
| Protein Name | Dna Repair And Recombination Protein Rad54-LikeRad54 HomologHhr54Hrad54 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna Repair And Recombination Protein Rad54-Like antibodyAnti-Rad54 Homolog antibodyAnti-Hhr54 antibodyAnti-Hrad54 antibodyAnti-RAD54L antibodyAnti-RAD54A antibody |
Information sourced from Uniprot.org
