• Western blot analysis of extracts of various cell lines, using Rad52 Rabbit monoclonal antibody (STJ11102287) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-RAD52 antibody (319-418) [S7MR] (STJ11102287)

SKU:
STJ11102287

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Rad52 (319-418) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAD52
Gene ID: 5893
Uniprot ID: RAD52_HUMAN
Immunogen Region: 319-418
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 319-418 of human Rad52 (P43351).
Immunogen Sequence: QELIKTLEDNSEKWAVTPDA GDGVVKPSSRADPAQTSDTL ALNNQMVTQNRTPHSVCHQK PQAKSGSWDLQTYSADQRTT GNWESHRKSQDMKKRKYDPS
Post Translational Modifications Phosphorylated upon DNA damage by ABL1, and probably by ATM or ATR.
Function Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
Protein Name Dna Repair Protein Rad52 Homolog
Database Links Reactome: R-HSA-3108214
Reactome: R-HSA-5685938
Reactome: R-HSA-5685939
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna Repair Protein Rad52 Homolog antibody
Anti-RAD52 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance