| Host: | Mouse |
| Applications: | WB/ICC/IHC |
| Reactivity: | Human/Mouse/Rat/Monkey/Hamster |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-UV excision repair protein RAD23 homolog B is suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 5H1-A10-A7 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2b |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000ICC 1:100 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RAD23B |
| Gene ID: | 5887 |
| Uniprot ID: | RD23B_HUMAN |
| Specificity: | This antibody detects endogenous levels of hHR23b and does not cross-react with related proteins. |
| Immunogen: | Purified recombinant human hHR23b protein fragments expressed in E.coli. |
| Protein Name | Uv Excision Repair Protein Rad23 Homolog BHr23bHhr23bXp-C Repair-Complementing Complex 58 Kda ProteinP58 |
| Database Links | Reactome: R-HSA-532668Reactome: R-HSA-5689877Reactome: R-HSA-5696394Reactome: R-HSA-5696395 |
| Cellular Localisation | NucleusCytoplasmThe Intracellular Distribution Is Cell Cycle DependentLocalized To The Nucleus And The Cytoplasm During G1 PhaseNuclear Levels Decrease During S-PhaseUpon Entering MitosisRelocalizes In The Cytoplasm Without Association With Chromatin |
| Alternative Antibody Names | Anti-Uv Excision Repair Protein Rad23 Homolog B antibodyAnti-Hr23b antibodyAnti-Hhr23b antibodyAnti-Xp-C Repair-Complementing Complex 58 Kda Protein antibodyAnti-P58 antibodyAnti-RAD23B antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and Rad23B knockout (KO) HeLa cells, using [KO Validated] Rad23B Rabbit pAb (STJ11100876) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89810/405584/STJ11100876_1__82734.1744891351.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Rad23B knockout (KO) HeLa cells, using [KO Validated] Rad23B Rabbit pAb (STJ11100876) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.")
