![Western blot analysis of lysates from wild type (WT) and Rad23A knockout (KO) 293T cells, using [KO Validated] Rad23A Rabbit polyclonal antibody (STJ11100760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89697/358182/STJ11100760_1__74942.1713122493.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Rad23A knockout (KO) 293T cells, using [KO Validated] Rad23A Rabbit polyclonal antibody (STJ11100760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and Rad23A knockout (KO) 293T cells, using [KO Validated] Rad23A Rabbit polyclonal antibody (STJ11100760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89697/358182/STJ11100760_1__74942.1713122493.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Rad23A knockout (KO) 293T cells, using [KO Validated] Rad23A Rabbit polyclonal antibody (STJ11100760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Short Description : | Rabbit polyclonal Rad23A (1-363) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RAD23A |
| Gene ID: | 5886 |
| Uniprot ID: | RD23A_HUMAN |
| Immunogen Region: | 1-363 |
| Immunogen Sequence: | MAVTITLKTLQQQTFKIRME PDETVKVLKEKIEAEKGRDA FPVAGQKLIYAGKILSDDVP IRDYRIDEKNFVVVMVTKTK AGQGTSAPPEASPTAAPESS TSFPPAPTSGMSHPPPAARE DKSPSEESAPTTSPESVSGS VPSSGSSGREEDAASTLVTG SEYETMLTEIMSMGYERERV VAALRASYNNPHRAVEYLLT GIPGSPEPEHGSVQESQVSE QPATEAAGENPLEFLRDQP |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-363 of human Rad23A (NP_005044.1). |
| Function | Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC. (Microbial infection) Involved in Vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 Vpr with the host proteasome. |
| Protein Name | Uv Excision Repair Protein Rad23 Homolog AHr23aHhr23a |
| Database Links | Reactome: R-HSA-5689877Reactome: R-HSA-5696394Reactome: R-HSA-5696395 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Uv Excision Repair Protein Rad23 Homolog A antibodyAnti-Hr23a antibodyAnti-Hhr23a antibodyAnti-RAD23A antibody |
Information sourced from Uniprot.org
