Host: | Rabbit |
Applications: | WB/IHC/IF/ELISA |
Reactivity: | Human/Mouse |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-Cell cycle checkpoint protein RAD17 (621-670 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration: | 1 mg/mL |
Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:10000 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | RAD17 |
Gene ID: | 5884 |
Uniprot ID: | RAD17_HUMAN |
Immunogen Region: | 621-670 aa |
Specificity: | Rad17 Polyclonal Antibody detects endogenous levels of Rad17 protein. |
Immunogen: | The antiserum was produced against synthesized peptide derived from the human RAD17 at the amino acid range 621-670 |
Post Translational Modifications | Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4. Phosphorylation at Thr-633 by ATM in response to DNA damage promotes interaction with NBN and recruitment of the MRN complex to DNA damage sites. |
Function | Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the 9-1-1 (RAD1-RAD9-HUS1) complex and RHNO1 onto chromatin, and in CHEK1 activation. Involved in homologous recombination by mediating recruitment of the MRN complex to DNA damage sites. May also serve as a sensor of DNA replication progression. |
Protein Name | Cell Cycle Checkpoint Protein Rad17 Hrad17 Rf-C/Activator 1 Homolog |
Database Links | Reactome: R-HSA-176187 Reactome: R-HSA-5685938 Reactome: R-HSA-5693607 Reactome: R-HSA-5693616 Reactome: R-HSA-6804756 Reactome: R-HSA-69473 Reactome: R-HSA-9709570 |
Cellular Localisation | Nucleus Chromosome Phosphorylated Form Redistributes To Discrete Nuclear Foci Upon Dna Damage Localizes To Dna Double-Strand Breaks (Dsbs) |
Alternative Antibody Names | Anti-Cell Cycle Checkpoint Protein Rad17 antibody Anti-Hrad17 antibody Anti-Rf-C/Activator 1 Homolog antibody Anti-RAD17 antibody Anti-R24L antibody |
Information sourced from Uniprot.org