Anti-RAD1 antibody (STJ193200)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human RAD1
General Information
| Short Description | Rabbit polyclonal anti-Cell cycle checkpoint protein RAD1 for use in IHC, IF and WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC-P 1:50-200WB 1:500-2000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAD1 |
| Gene ID | 5810 |
| Uniprot ID | RAD1_HUMAN |
| Immunogen | Synthesized peptide derived from the human RAD1 |
| Specificity | This antibody detects endogenous levels of human RAD1 |
Additional Info
| Function | Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds.endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths.and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. |
| Protein Name | Cell Cycle Checkpoint Protein Rad1Hrad1Rad1-Like Dna Damage Checkpoint Protein |
| Database Links | Reactome: R-HSA-176187Reactome: R-HSA-5685938Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9709570 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Cell Cycle Checkpoint Protein Rad1 antibodyAnti-Hrad1 antibodyAnti-Rad1-Like Dna Damage Checkpoint Protein antibodyAnti-RAD1 antibodyAnti-REC1 antibody |
Information sourced from Uniprot.org