• STJ70121 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ70121 (1µg/ml) staining of A431 nuclear (A) , Jurkat (B) , Jurkat nuclear (C) and negative control Human Pancreas (D) lysate. (35µg protein in RIPA buffer) Detected by chemiluminescence.
  • STJ70121 (4µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
  • STJ70121 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-RACGAP1/MgcRacGAP antibody (C-Term) (STJ70121)

SKU:
STJ70121-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-RACGAP1/MgcRacGAP (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IHC-1µg/ml
IF-Strong expression of the protein seen in the cytoplasm of MCF7 cells. 10µg/ml
FC-Flow cytometric analysis of MCF7 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: RACGAP1
Gene ID: 29127
Uniprot ID: RGAP1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_037409.2; NP_001306934.1; NP_001306935.1; NP_001306936.1
Specificity: This antibody is expected to recognize all reported isoforms (NP_037409.2; NP_001306934.1; NP_001306935.1; NP_001306936.1). Reported variants represent identical protein: NP_001306928.1, NP_001119576.1, NP_037409.2, NP_001306931.1, NP_001306929.1, NP
Immunogen Sequence: GRQGNFFASPMLK
Post Translational Modifications Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation. Phosphorylation on Ser-164 plays a major role in mediating interaction with ECT2. Phosphorylation on Ser-157 does not appear to contribute to binding to ECT2.
Function Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Has a critical role in erythropoiesis. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
Protein Name Rac Gtpase-Activating Protein 1
Male Germ Cell Racgap
Mgcracgap
Protein Cyk4 Homolog
Cyk4
Hscyk-4
Database Links Reactome: R-HSA-2132295
Reactome: R-HSA-6811434
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013405
Reactome: R-HSA-9013423
Reactome: R-HSA-983189
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Spindle
Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Cleavage Furrow
Midbody
Midbody Ring
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Colocalizes With Rnd2 In Golgi-Derived Proacrosomal Vesicles And The Acrosome
During Interphase
Localized To The Nucleus And Cytoplasm Along With Microtubules
In Anaphase
Is Redistributed To The Central Spindle And
In Telophase And Cytokinesis
To The Midbody Ring
Also Called Flemming Body
Colocalizes With Rhoa At The Myosin Contractile Ring During Cytokinesis
Colocalizes With Ect2 To The Mitotic Spindles During Anaphase/Metaphase
The Cleavage Furrow During Telophase And At The Midbody At The End Of Cytokinesis
Colocalizes With Cdc42 To Spindle Microtubules From Prometaphase To Telophase
Alternative Antibody Names Anti-Rac Gtpase-Activating Protein 1 antibody
Anti-Male Germ Cell Racgap antibody
Anti-Mgcracgap antibody
Anti-Protein Cyk4 Homolog antibody
Anti-Cyk4 antibody
Anti-Hscyk-4 antibody
Anti-RACGAP1 antibody
Anti-KIAA1478 antibody
Anti-MGCRACGAP antibody

Information sourced from Uniprot.org

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