Anti-RAC3 antibody (80-160 aa) (STJ191267)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 80-160
General Information
| Short Description | Rabbit polyclonal anti-Ras-related C3 botulinum toxin substrate 3 (80-160 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAC3 |
| Gene ID | 5881 |
| Uniprot ID | RAC3_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 80-160 |
| Immunogen Region | 80-160 aa |
| Specificity | RAC3 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | (Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly. Ubiquitinated at Lys-166 in a FBXL19-mediated manner.leading to proteasomal degradation. |
| Function | Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner. |
| Protein Name | Ras-Related C3 Botulinum Toxin Substrate 3P21-Rac3 |
| Database Links | Reactome: R-HSA-4086400Reactome: R-HSA-9013423 |
| Cellular Localisation | CytoplasmEndomembrane SystemCell ProjectionLamellipodiumPerinuclear RegionCell MembraneCytoskeletonMembrane-Associated When ActivatedColocalizes With Nrbp To Endomembranes And At The Cell Periphery In LamellipodiaColocalized With Cib1 In The Perinuclear Area And At The Cell Periphery |
| Alternative Antibody Names | Anti-Ras-Related C3 Botulinum Toxin Substrate 3 antibodyAnti-P21-Rac3 antibodyAnti-RAC3 antibody |
Information sourced from Uniprot.org