| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RAC3 |
| Gene ID: | 5881 |
| Uniprot ID: | RAC3_HUMAN |
| Immunogen Region: | 1-192 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human RAC3 (NP_005043.1). |
| Immunogen Sequence: | MQAIKCVVVGDGAVGKTCLL ISYTTNAFPGEYIPTVFDNY SANVMVDGKPVNLGLWDTAG QEDYDRLRPLSYPQTDVFLI CFSLVSPASFENVRAKWYPE VRHHCPHTPILLVGTKLDLR DDKDTIERLRDKKLAPITYP QGLAMAREIGSVKYLECSAL TQRGLKTVFDEAIRAVLCPP PVKKPGKKCTVF |
| Tissue Specificity | Highest levels in brain, also detected in heart, placenta and pancreas. |
| Post Translational Modifications | (Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly. Ubiquitinated at Lys-166 in a FBXL19-mediated manner.leading to proteasomal degradation. |
| Function | Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner. |
| Protein Name | Ras-Related C3 Botulinum Toxin Substrate 3 P21-Rac3 |
| Database Links | Reactome: R-HSA-4086400 Reactome: R-HSA-9013423 |
| Cellular Localisation | Cytoplasm Endomembrane System Cell Projection Lamellipodium Perinuclear Region Cell Membrane Cytoskeleton Membrane-Associated When Activated Colocalizes With Nrbp To Endomembranes And At The Cell Periphery In Lamellipodia Colocalized With Cib1 In The Perinuclear Area And At The Cell Periphery |
| Alternative Antibody Names | Anti-Ras-Related C3 Botulinum Toxin Substrate 3 antibody Anti-P21-Rac3 antibody Anti-RAC3 antibody |
Information sourced from Uniprot.org
