| Background | The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen.An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. |
Information sourced from Uniprot.org
![Confocal imaging of HeLa cells using [KD Validated] TIMM10 Rabbit mAb (STJ248506, dilution 1:200) followed by a further incubation with ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L) (STJS001196, dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse mAb (STJ11107556, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (STJS001209, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/185065/412429/STJS001196_1__48336.1744898801.jpg?c=1 "Confocal imaging of HeLa cells using [KD Validated] TIMM10 Rabbit mAb (STJ248506, dilution 1:200) followed by a further incubation with ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L) (STJS001196, dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse mAb (STJ11107556, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (STJS001209, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.")
