• Western blot analysis of extracts of various cell lines, using RAB5C antibody (STJ29481) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-RAB5C antibody (1-216) (STJ29481)

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STJ29481

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB5C (1-216) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB5C
Gene ID: 5878
Uniprot ID: RAB5C_HUMAN
Immunogen Region: 1-216
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-216 of human RAB5C (NP_958842.1).
Immunogen Sequence: MAGRGGAARPNGPAAGNKIC QFKLVLLGESAVGKSSLVLR FVKGQFHEYQESTIGAAFLT QTVCLDDTTVKFEIWDTAGQ ERYHSLAPMYYRGAQAAIVV YDITNTDTFARAKNWVKELQ RQASPNIVIALAGNKADLAS KRAVEFQEAQAYADDNSLLF METSAKTAMNVNEIFMAIAK KLPKNEPQNATGAPGRNRGV DLQENNPASRSQCCSN
Post Translational Modifications Phosphorylation of Ser-85 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2. (Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3.
Function Protein transport. Probably involved in vesicular traffic.
Protein Name Ras-Related Protein Rab-5c
L1880
Rab5l
Database Links Reactome: R-HSA-432722
Reactome: R-HSA-6798695
Reactome: R-HSA-8854214
Reactome: R-HSA-8856828
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Early Endosome Membrane
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Ras-Related Protein Rab-5c antibody
Anti-L1880 antibody
Anti-Rab5l antibody
Anti-RAB5C antibody
Anti-RABL antibody

Information sourced from Uniprot.org

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