| Short Description : | Goat polyclonal RAB27A, member RAS oncogene family (120aa C-Term) antibody for use in WB, IF, IHC-P and IHC-F in human, mouse, rat, bovine, canine, chicken, avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep and simian sa |
| Applications: | WB/IF/IHC-P/IHC-F |
| Host: | Goat |
| Reactivity: | Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range: | WB 1:250-1:2000IF 1:50-1:200IHC-P 1:50-1:400IHC-F 1:50-1:400Alzahofi N Welz T Robinson CL et al. Nat Commun. 2020 Jul PMID: 32661310Ramsay E Raviña M Sarkhel S et al. Pharmaceutics 2020 Jul PMID: 32708811Sanzà P Evans RD |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction: | Store at-20 C for long-term storage. Store at 2-8 C for up to one month. |
| Gene Symbol: | RAB27A |
| Gene ID: | 5873 |
| Uniprot ID: | RB27A_HUMAN |
| Accession Number: | ENSG00000069974 |
| Immunogen: | Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab27a produced in E. coli. |
| Immunogen Region: | 120aa C-Term |
| Immunogen Sequence: | MHAYCENPDIVLCGNKSDLE HAYCENPDIVLCGNKSDLED AYCENPDIVLCGNKSDLEDQ YCENPDIVLCGNKSDLEDQR CENPDIVLCGNKSDLEDQRA ENPDIVLCGNKSDLEDQRAV |
| Specificity: | Detects Rab27a by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a. This antibody also recognizes Rab27b protein. |
| Tissue Specificity | Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells. |
| Function | Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. |
| Protein Name | Ras-Related Protein Rab-27aRab-27Gtp-Binding Protein Ram |
| Database Links | Reactome: R-HSA-264876Reactome: R-HSA-6798695Reactome: R-HSA-8873719Reactome: R-HSA-8876198Reactome: R-HSA-9824585 |
| Cellular Localisation | MembraneLipid-AnchorMelanosomeLate EndosomeLysosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvLocalizes To Endosomal Exocytic Vesicles |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-27a antibodyAnti-Rab-27 antibodyAnti-Gtp-Binding Protein Ram antibodyAnti-RAB27A antibodyAnti-RAB27 antibody |
Information sourced from Uniprot.org
