• Immunofluorescence analysis of L929 cells using RAB20 Rabbit polyclonal antibody (STJ119767) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse heart, using RAB20 Rabbit polyclonal antibody (STJ119767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-RAB20 antibody (80-190) (STJ119767)

SKU:
STJ119767

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB20 (80-190) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB20
Gene ID: 55647
Uniprot ID: RAB20_HUMAN
Immunogen Region: 80-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-190 of human RAB20 (NP_060287.1).
Immunogen Sequence: YDVNHRQSLVELEDRFLGLT DTASKDCLFAIVGNKVDLTE EGALAGQEKEECSPNMDAGD RVSPRAPKQVQLEDAVALYK KILKYKMLDEQDVPAAEQMC FETSAKTGYNV
Tissue Specificity Low or absent expression in normal pancreas and stronger expression in 15 of 18 exocrine pancreatic adenocarcinomas (at protein level).
Function Plays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes.
Protein Name Ras-Related Protein Rab-20
Database Links Reactome: R-HSA-8873719
Cellular Localisation Golgi Apparatus
Cytoplasmic Vesicle
Phagosome
Phagosome Membrane
Lipid-Anchor
Cytoplasmic Side
Highly Enriched On Apical Endocytic Structures In Polarized Epithelial Cells Of Kidney Proximal Tubules
Recruited To Phagosomes Containing S
Aureus Or M
Tuberculosis
Alternative Antibody Names Anti-Ras-Related Protein Rab-20 antibody
Anti-RAB20 antibody

Information sourced from Uniprot.org

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