| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RAB13 (1-203) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RAB13 |
| Gene ID: |
5872 |
| Uniprot ID: |
RAB13_HUMAN |
| Immunogen Region: |
1-203 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-203 of human RAB13 (NP_002861.1). |
| Immunogen Sequence: |
MAKAYDHLFKLLLIGDSGVG KTCLIIRFAEDNFNNTYIST IGIDFKIRTVDIEGKKIKLQ VWDTAGQERFKTITTAYYRG AMGIILVYDITDEKSFENIQ NWMKSIKENASAGVERLLLG NKCDMEAKRKVQKEQADKLA REHGIRFFETSAKSSMNVDE AFSSLARDILLKSGGRRSGN GNKPPSTDLKTCDKKNTNKC SLG |
| Tissue Specificity | Detected in several types of epithelia, including intestine, kidney, liver and in endothelial cells. |
| Post Translational Modifications | (Microbial infection) Stearoylated By S.flexneri N-epsilon-fatty acyltransferase IcsB, thereby disrupting the host actin cytoskeleton. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis. |
| Protein Name | Ras-Related Protein Rab-13Cell Growth-Inhibiting Gene 4 Protein |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-8873719Reactome: R-HSA-8876198 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideCytoplasmic Vesicle MembraneCell JunctionTight JunctionGolgi ApparatusTrans-Golgi Network MembraneRecycling Endosome MembraneCell ProjectionLamellipodiumTight Junctions Or Associated With Vesicles Scattered Throughout The Cytoplasm In Cells Lacking Tight JunctionsRelocalizes To The Leading Edge Of Lamellipodia In Migrating Endothelial Cells |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-13 antibodyAnti-Cell Growth-Inhibiting Gene 4 Protein antibodyAnti-RAB13 antibodyAnti-GIG4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
