| Host: |
Rabbit |
| Applications: |
IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Rab11 family-interacting protein 3 (569-618 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RAB11FIP3 |
| Gene ID: |
9727 |
| Uniprot ID: |
RFIP3_HUMAN |
| Immunogen Region: |
569-618 aa |
| Specificity: |
Rab11-FIP3 Polyclonal Antibody detects endogenous levels of Rab11-FIP3 protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human RAB11FIP3 at the amino acid range 569-618 |
| Function | Downstream effector molecule for Rab11 GTPase which acts as a regulator of endocytic trafficking and intracellular ciliogenesis by participating in membrane delivery. Recruited by Rab11 to endosomes where it links Rab11 to dynein motor complex. The functional Rab11-RAB11FIP3-dynein complex then regulates the movement of peripheral sorting endosomes (SE) along microtubule tracks toward the microtubule organizing center/centrosome, generating the endocytic recycling compartment (ERC) during interphase of cell cycle. Facilitates the interaction between dynein and dynactin and activates dynein processivity. Binding with ASAP1 is also needed to regulates the pericentrosomal localization of recycling endosomes. The Rab11-RAB11FIP3 complex is also implicated in the transport and fusion of vesicles derived from recycling endosomes to the cleavage furrow during late cytokinesis and abscission. Also regulates cleavage furrow ingression and abscission together with RACGAP1. Required for ARF6 recruitment to the cleavage furrow during cytokinesis. Involved in the Rab11-Rabin8-Rab8 ciliogenesis cascade by facilitating the orderly assembly of a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, which direct preciliary vesicle trafficking to mother centriole and ciliogenesis initiation. Also promotes the activity of Rab11 and ASAP1 in the ARF4-dependent Golgi-to-cilia transport of the sensory receptor rhodopsin. Competes with WDR44 for binding to Rab11, which controls intracellular ciliogenesis pathway. May play a role in breast cancer cell motility by regulating actin cytoskeleton. |
| Protein Name | Rab11 Family-Interacting Protein 3Fip3Fip3-Rab11Rab11-Fip3Arfophilin-1Ef Hands-Containing Rab-Interacting ProteinEferinMu-Mb-17.148 |
| Database Links | Reactome: R-HSA-5620916 |
| Cellular Localisation | Endosome MembraneRecycling Endosome MembranePeripheral Membrane ProteinCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCleavage FurrowMidbodyGolgi Apparatus MembraneGolgi ApparatusTrans-Golgi Network MembraneDuring InterphaseLocalized In Vesicles Continuously Moving From Peripheral Sorting Endosomes In The Cell Towards The Pericentrosomal Endosomal Recycling Compartment (Erc)In Early Mitosis Remains Diffuse And Distributed Through The CellThe Onset Of Anaphase Sequesters These Vesicles To The Centrosomes At The Opposite Poles Of The CellDuring Telophase These Vesicles Move From The CentrosomesTo The FurrowAnd Then To The Midbody To Aid In AbscissionInteraction With Rab11 Mediates Localization To EndosomesInteraction With Arf6 Mediates Localization To The MidbodyLocalized To The Golgi And Tgn When Interacting With Rho In PhotoreceptorsLocalized To Rhodopsin Transport Carriers When Interacting With Rab11a And Asap1 In Photoreceptors |
| Alternative Antibody Names | Anti-Rab11 Family-Interacting Protein 3 antibodyAnti-Fip3 antibodyAnti-Fip3-Rab11 antibodyAnti-Rab11-Fip3 antibodyAnti-Arfophilin-1 antibodyAnti-Ef Hands-Containing Rab-Interacting Protein antibodyAnti-Eferin antibodyAnti-Mu-Mb-17.148 antibodyAnti-RAB11FIP3 antibodyAnti-ARFO1 antibodyAnti-KIAA0665 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
