| Host: | Goat |
| Applications: | Pep-ELISA/FC/IHC |
| Reactivity: | Human/Mouse/Rat/Dog/Cow/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Pyruvate Carboxylase (Internal) is suitable for use in ELISA, Flow Cytometry and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.IHC: Paraffin embedded Human Liver. Recommended concentration: 5µg/ml.FC:Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | PC |
| Gene ID: | 5091 |
| Uniprot ID: | PYC_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_000911.2; NP_001035806.1; NP_071504.2 |
| Specificity: | All reported variants (NP_000911.2; NP_001035806.1; NP_071504.2) represent identical protein. |
| Immunogen Sequence: | KFKEVKKAYVEANQ |
| Function | Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. |
| Protein Name | Pyruvate Carboxylase - MitochondrialPyruvic CarboxylasePcb |
| Database Links | Reactome: R-HSA-196780Reactome: R-HSA-3371599Reactome: R-HSA-70263Reactome: R-HSA-70268 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Carboxylase - Mitochondrial antibodyAnti-Pyruvic Carboxylase antibodyAnti-Pcb antibodyAnti-PC antibody |
Information sourced from Uniprot.org