• Western blot analysis of extracts of various cell lines, using PYGB antibody (STJ28485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of HepG2 cells using PYGB rabbit polyclonal antibody (STJ28485) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using PYGB rabbit polyclonal antibody (STJ28485) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using PYGB rabbit polyclonal antibody (STJ28485) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-3 cells using PYGB rabbit polyclonal antibody (STJ28485) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells, using 3 Mu g PYGB antibody (STJ28485). Western blot was performed from the immunoprecipitate using PYGB antibody (STJ28485) at a dilution of 1:1000.

Anti-PYGB antibody (544-843) (STJ28485)

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STJ28485

€140.63 - €655.63
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Host: Rabbit
Applications: WB/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PYGB
Gene ID: 5834
Uniprot ID: PYGB_HUMAN
Immunogen Region: 544-843
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 544-843 of human PYGB (NP_002853.2).
Immunogen Sequence: LKFSAFLEKEYKVKINPSSM FDVHVKRIHEYKRQLLNCLH VVTLYNRIKRDPAKAFVPRT VMIGGKAAPGYHMAKLIIKL VTSIGDVVNHDPVVGDRLKV IFLENYRVSLAEKVIPAADL SQQISTAGTEASGTGNMKFM LNGALTIGTMDGANVEMAEE AGAENLFIFGLRVEDVEALD RKGYNAREYYDHLPELKQAV DQISSGFFSPKEPDCFKDIV NMLMHHDRFKVFADYEAYM
Post Translational Modifications Phosphorylated. Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
Function Glycogen phosphorylase that regulates glycogen mobilization. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
Protein Name Glycogen Phosphorylase - Brain Form
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-70221
Alternative Antibody Names Anti-Glycogen Phosphorylase - Brain Form antibody
Anti-PYGB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance