| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Apoptosis-Associated Speck-Like Protein Containing A Card (4-110) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S5198RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:10000-1:20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PYCARD |
| Gene ID: |
29108 |
| Uniprot ID: |
ASC_HUMAN |
| Immunogen Region: |
4-110 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 4-110 of human ASC/TMS1 (NP_037390.2). |
| Immunogen Sequence: |
ARDAILDALENLTAEELKKF KLKLLSVPLREGYGRIPRGA LLSMDALDLTDKLVSFYLET YGAELTANVLRDMGLQEMAG QLQAATHQGSGAAPAGIQAP PQSAAKP |
| Tissue Specificity | Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Expressed in lung epithelial cells (at protein level). Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. |
| Post Translational Modifications | Phosphorylated. 'Lys-63'-linked polyubiquitination by TRAF3 is critical for speck formation and inflammasome activation. |
| Function | Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in innate immune response by acting as an integral adapter in the assembly of various inflammasomes (NLRP1, NLRP2, NLRP3, NLRP6, AIM2 and probably IFI16) which recruit and activate caspase-1 leading to processing and secretion of pro-inflammatory cytokines. Caspase-1-dependent inflammation leads to macrophage pyroptosis, a form of cell death. The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions. Clustered PYCARD nucleates the formation of caspase-1 filaments through the interaction of their respective CARD domains, acting as a platform for of caspase-1 polymerization. In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1. In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation. May be involved in RIGI-triggered pro-inflammatory responses and inflammasome activation. In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8. In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2.the latter function is proposed to involve the nuclear form. Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome.this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways. For regulation of NF-kappa-B activating and inhibiting functions have been reported. Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK. Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing. Modulates host resistance to DNA virus infection, probably by inducing the cleavage of and inactivating CGAS in presence of cytoplasmic double-stranded DNA. Isoform 2: May have a regulating effect on the function as inflammasome adapter. Isoform 3: Seems to inhibit inflammasome-mediated maturation of interleukin-1 beta. |
| Protein Name | Apoptosis-Associated Speck-Like Protein Containing A CardHascCaspase Recruitment Domain-Containing Protein 5Pyd And Card Domain-Containing ProteinTarget Of Methylation-Induced Silencing 1 |
| Database Links | Reactome: R-HSA-5660668Reactome: R-HSA-6798695Reactome: R-HSA-844456Reactome: R-HSA-844615Reactome: R-HSA-9660826Reactome: R-HSA-9692916 |
| Cellular Localisation | CytoplasmInflammasomeEndoplasmic ReticulumMitochondrionNucleusUpstream Of Caspase ActivationA Redistribution From The Cytoplasm To The Aggregates OccursThese Appear As HollowPerinuclear SphericalBall-Like StructuresUpon Nlrp3 Inflammasome Activation Redistributes To The Perinuclear Space Localizing To Endoplasmic Reticulum And MitochondriaLocalized Primarily To The Nucleus In Resting Monocytes/Macrophages And Rapidly Redistributed To The Cytoplasm Upon Pathogen InfectionLocalized To Large Cytoplasmic Aggregate Appearing As A Speck Containing Aim2PycardCasp8 And Bacterial Dna After Infection With Francisella TularensisGolgi Apparatus Membrane(Microbial Infection) Upon Hrsv InfectionThe Protein Is Mainly Located In Lipid Rafts In The Golgi Membrane |
| Alternative Antibody Names | Anti-Apoptosis-Associated Speck-Like Protein Containing A Card antibodyAnti-Hasc antibodyAnti-Caspase Recruitment Domain-Containing Protein 5 antibodyAnti-Pyd And Card Domain-Containing Protein antibodyAnti-Target Of Methylation-Induced Silencing 1 antibodyAnti-PYCARD antibodyAnti-ASC antibodyAnti-CARD5 antibodyAnti-TMS1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
