• Immunohistochemistry analysis of PTPRN2 in paraffin-embedded mouse pancreas using PTPRN2 Rabbit polyclonal antibody (STJ116974) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PTPRN2 in paraffin-embedded human lung cancer using PTPRN2 Rabbit polyclonal antibody (STJ116974) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PTPRN2 in paraffin-embedded human colon carcinoma using PTPRN2 Rabbit polyclonal antibody (STJ116974) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PTPRN2 in paraffin-embedded human mammary cancer using PTPRN2 Rabbit polyclonal antibody (STJ116974) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PTPRN2 Rabbit polyclonal antibody (STJ116974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PTPRN2 antibody (22-242) (STJ116974)

SKU:
STJ116974

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PTPRN2 (22-242) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTPRN2
Gene ID: 5799
Uniprot ID: PTPR2_HUMAN
Immunogen Region: 22-242
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-242 of human PTPRN2 (NP_570858.2).
Immunogen Sequence: AAPSSVPRGRQLPGRLGCLL EEGLCGASEACVNDGVFGRC QKVPAMDFYRYEVSPVALQR LRVALQKLSGTGFTWQDDYT QYVMDQELADLPKTYLRRPE ASSPARPSKHSVGSERRYSR EGGAALANALRRHLPFLEAL SQAPASDVLARTHTAQDRPP AEGDDRFSESILTYVAHTSA LTYPPGSRTQLREDLLPRTL GQLQPDELSPKVDSGVDRHH L
Tissue Specificity Highest levels in brain and pancreas. Lower levels in trachea, prostate, stomach and spinal cord.
Post Translational Modifications Subject to proteolytic cleavage at multiple sites.
Function Plays a role in vesicle-mediated secretory processes. Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets. Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation. Plays a role in insulin secretion in response to glucose stimuli. Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain. In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Required to maintain normal levels of renin expression and renin release. May regulate catalytic active protein-tyrosine phosphatases such as PTPRA through dimerization. Has phosphatidylinositol phosphatase activity.the PIPase activity is involved in its ability to regulate insulin secretion. Can dephosphorylate phosphatidylinositol 4,5-biphosphate (PI(4,5)P2), phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate. Regulates PI(4,5)P2 level in the plasma membrane and localization of cofilin at the plasma membrane and thus is indirectly involved in regulation of actin dynamics related to cell migration and metastasis.upon hydrolyzation of PI(4,5)P2 cofilin is released from the plasma membrane and acts in the cytoplasm in severing F-actin filaments.
Protein Name Receptor-Type Tyrosine-Protein Phosphatase N2
R-Ptp-N2
Islet Cell Autoantigen-Related Protein
Iar
Icaar
Phogrin Cleaved Into - Ia-2beta60
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle Membrane
Single-Pass Type I Membrane Protein
Secretory Vesicle
Synaptic Vesicle Membrane
Predominantly Found On Dense-Core Secretory Granules
Sorting To Secretory Granules In Part Is Dependent Of The N-Terminal Propeptide Domain Of The Precursor And Its Interaction With Cpe
Transiently Found At The Cell Membrane
When Secretory Vesicles Fuse With The Cell Membrane To Release Their Cargo
Is Then Endocytosed And Recycled To Secretory Vesicles Involving Clathrin-Dependent Ap2-Mediated Endocytosis
Recycled Via Stx6- But Not Tttgn1/Tgn38-Containing Compartments
Ia-2beta60: Cytoplasmic Vesicle
Alternative Antibody Names Anti-Receptor-Type Tyrosine-Protein Phosphatase N2 antibody
Anti-R-Ptp-N2 antibody
Anti-Islet Cell Autoantigen-Related Protein antibody
Anti-Iar antibody
Anti-Icaar antibody
Anti-Phogrin Cleaved Into - Ia-2beta60 antibody
Anti-PTPRN2 antibody
Anti-KIAA0387 antibody

Information sourced from Uniprot.org

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