| Host: | Rabbit |
| Applications: | ELISA/IHC/IP/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-PTPRA (650-700) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.5-1.5 µg/µl |
| Dilution Range: | WB: 1:2, 500-1:5, 000 ELISA: 1:50, 000 IP: 1:200 IHC: 1:50-1:250 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | PTPRA |
| Gene ID: | 5786 |
| Uniprot ID: | PTPRA_HUMAN |
| Immunogen Region: | 650-700 |
| Immunogen: | Synthetic peptide taken within amino acid region 650-700 on human Receptor-type tyrosine-protein phosphatase Alpha protein. |
| Post Translational Modifications | Integrin binding to extracellular matrix induces phosphorylation at Tyr-798 which induces PTPRA localization and recruitment of BCAR3, BCAR1 and CRK to focal adhesions. |
| Function | Tyrosine protein phosphatase which is involved in integrin-mediated focal adhesion formation. Following integrin engagement, specifically recruits BCAR3, BCAR1 and CRK to focal adhesions thereby promoting SRC-mediated phosphorylation of BRAC1 and the subsequent activation of PAK and small GTPase RAC1 and CDC42. |
| Protein Name | Receptor-Type Tyrosine-Protein Phosphatase Alpha Protein-Tyrosine Phosphatase Alpha R-Ptp-Alpha |
| Database Links | Reactome: R-HSA-375165 Reactome: R-HSA-5673001 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Cell Junction Focal Adhesion Localizes To Focal Adhesion Sites Following Integrin Engagement |
| Alternative Antibody Names | Anti-Receptor-Type Tyrosine-Protein Phosphatase Alpha antibody Anti-Protein-Tyrosine Phosphatase Alpha antibody Anti-R-Ptp-Alpha antibody Anti-PTPRA antibody Anti-PTPA antibody Anti-PTPRL2 antibody |
Information sourced from Uniprot.org