| Host: |
Mouse |
| Applications: |
WB/ICC/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-SHP1 (C-Term) is suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
M160 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:500ICC 1:100IP 1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PTPN6 |
| Gene ID: |
5777 |
| Uniprot ID: |
PTN6_HUMAN |
| Immunogen Region: |
C-Term |
| Specificity: |
The antibody detects a 68 kDa* protein in human A431 and Jurkat cells, and does not cross-react with SHP2. |
| Immunogen: |
Clone (M160) was generated from a recombinant protein containing amino acids in the C-terminal region of human SHP1. This sequence is highly conserved in rat and mouse SHP1. |
| Post Translational Modifications | Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation. Phosphorylation at Tyr-564 by LYN enhances phosphatase activity. Phosphorylation at Thr-394 by TAOK3 leads to polyubiquitination and subsequent proteasomal degradation. Ubiquitinated after phosphorylation by TAOK3. Ubiquitinated by a cooperation between ITCH and WWP2 via 'Lys-27'-mediated polyubiquitin chains resulting in the reduction of its association with LCK. |
| Function | Tyrosine phosphatase enzyme that plays important roles in controlling immune signaling pathways and fundamental physiological processes such as hematopoiesis. Dephosphorylates and negatively regulate several receptor tyrosine kinases (RTKs) such as EGFR, PDGFR and FGFR, thereby modulating their signaling activities. When recruited to immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing receptors such as immunoglobulin-like transcript 2/LILRB1, programmed cell death protein 1/PDCD1, CD3D, CD22, CLEC12A and other receptors involved in immune regulation, initiates their dephosphorylation and subsequently inhibits downstream signaling events. Modulates the signaling of several cytokine receptors including IL-4 receptor. Additionally, targets multiple cytoplasmic signaling molecules including STING1, LCK or STAT1 among others involved in diverse cellular processes including modulation of T-cell activation or cGAS-STING signaling. Within the nucleus, negatively regulates the activity of some transcription factors such as NFAT5 via direct dephosphorylation. Acts also as a key transcriptional regulator of hepatic gluconeogenesis by controlling recruitment of RNA polymerase II to the PCK1 promoter together with STAT5A. |
| Protein Name | Tyrosine-Protein Phosphatase Non-Receptor Type 6Hematopoietic Cell Protein-Tyrosine PhosphataseProtein-Tyrosine Phosphatase 1cPtp-1cProtein-Tyrosine Phosphatase Shp-1Sh-Ptp1 |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-1433559Reactome: R-HSA-201556Reactome: R-HSA-210990Reactome: R-HSA-388841Reactome: R-HSA-389948Reactome: R-HSA-391160Reactome: R-HSA-432142Reactome: R-HSA-512988Reactome: R-HSA-5690714Reactome: R-HSA-6798695Reactome: R-HSA-877300Reactome: R-HSA-877312Reactome: R-HSA-9008059Reactome: R-HSA-909733Reactome: R-HSA-912526Reactome: R-HSA-912694Reactome: R-HSA-9705671Reactome: R-HSA-9725371Reactome: R-HSA-982772Reactome: R-HSA-983695 |
| Cellular Localisation | CytoplasmNucleusIn NeuronsTranslocates Into The Nucleus After Treatment With Angiotensin IiShuttles Between The Cytoplasm And Nucleus Via Its Association With Pdpk1 |
| Alternative Antibody Names | Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 6 antibodyAnti-Hematopoietic Cell Protein-Tyrosine Phosphatase antibodyAnti-Protein-Tyrosine Phosphatase 1c antibodyAnti-Ptp-1c antibodyAnti-Protein-Tyrosine Phosphatase Shp-1 antibodyAnti-Sh-Ptp1 antibodyAnti-PTPN6 antibodyAnti-HCP antibodyAnti-PTP1C antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
