• Immunohistochemistry analysis of paraffin-embedded mouse brain using PTPN23 Rabbit polyclonal antibody (STJ25227) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using PTPN23 Rabbit polyclonal antibody (STJ25227) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PTPN23 antibody (STJ25227) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PTPN23 antibody (1230-1450) (STJ25227)

SKU:
STJ25227

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PTPN23 (1230-1450) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTPN23
Gene ID: 25930
Uniprot ID: PTN23_HUMAN
Immunogen Region: 1230-1450
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1230-1450 of human PTPN23 (NP_056281.1).
Immunogen Sequence: DSNRVVLRSGKDDYINASCV EGLSPYCPPLVATQAPLPGT AADFWLMVHEQKVSVIVMLV SEAEMEKQKVARYFPTERGQ PMVHGALSLALSSVRSTETH VERVLSLQFRDQSLKRSLVH LHFPTWPELGLPDSPSNLLR FIQEVHAHYLHQRPLHTPII VHCSSGVGRTGAFALLYAAV QEVEAGNGIPELPQLVRRMR QQRKHMLQEKLHLRFCYEAV V
Function Plays a role in sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs) via its interaction with the ESCRT-I complex (endosomal sorting complex required for transport I), and possibly also other ESCRT complexes. May act as a negative regulator of Ras-mediated mitogenic activity. Plays a role in ciliogenesis.
Protein Name Tyrosine-Protein Phosphatase Non-Receptor Type 23
His Domain-Containing Protein Tyrosine Phosphatase
Hd-Ptp
Protein Tyrosine Phosphatase Td14
Ptp-Td14
Database Links Reactome: R-HSA-9008059
Cellular Localisation Nucleus
Cytoplasm
Cytoplasmic Vesicle
Endosome
Cytoskeleton
Cilium Basal Body
Early Endosome
Alternative Antibody Names Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 23 antibody
Anti-His Domain-Containing Protein Tyrosine Phosphatase antibody
Anti-Hd-Ptp antibody
Anti-Protein Tyrosine Phosphatase Td14 antibody
Anti-Ptp-Td14 antibody
Anti-PTPN23 antibody
Anti-KIAA1471 antibody

Information sourced from Uniprot.org

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