• Western blot analysis of various lysates, using PGHS-2 Rabbit polyclonal antibody (STJ11105324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-PTGS2 antibody (505-604) (STJ11105324)

SKU:
STJ11105324

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Prostaglandin G/H Synthase 2 (505-604) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTGS2
Gene ID: 5743
Uniprot ID: PGH2_HUMAN
Immunogen Region: 505-604
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 505-604 of human COX2/PTGS2 (P35354).
Immunogen Sequence: GETMVEVGAPFSLKGLMGNV ICSPAYWKPSTFGGEVGFQI INTASIQSLICNNVKGCPFT SFSVPDPELIKTVTINASSS RSGLDDINPTVLLKERSTEL
Post Translational Modifications S-nitrosylation by NOS2 (iNOS) activates enzyme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-526. Acetylated at Ser-565 by SPHK1. During neuroinflammation, acetylation by SPHK1 promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia.
Function Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate, AA, C20:4(n-6)), with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Similarly catalyzes successive cyclooxygenation and peroxidation of dihomo-gamma-linoleate (DGLA, C20:3(n-6)) and eicosapentaenoate (EPA, C20:5(n-3)) to corresponding PGH1 and PGH3, the precursors of 1- and 3-series prostaglandins. In an alternative pathway of prostanoid biosynthesis, converts 2-arachidonoyl lysophopholipids to prostanoid lysophopholipids, which are then hydrolyzed by intracellular phospholipases to release free prostanoids. Metabolizes 2-arachidonoyl glycerol yielding the glyceryl ester of PGH2, a process that can contribute to pain response. Generates lipid mediators from n-3 and n-6 polyunsaturated fatty acids (PUFAs) via a lipoxygenase-type mechanism. Oxygenates PUFAs to hydroperoxy compounds and then reduces them to corresponding alcohols. Plays a role in the generation of resolution phase interaction products (resolvins) during both sterile and infectious inflammation. Metabolizes docosahexaenoate (DHA, C22:6(n-3)) to 17R-HDHA, a precursor of the D-series resolvins (RvDs). As a component of the biosynthetic pathway of E-series resolvins (RvEs), converts eicosapentaenoate (EPA, C20:5(n-3)) primarily to 18S-HEPE that is further metabolized by ALOX5 and LTA4H to generate 18S-RvE1 and 18S-RvE2. In vascular endothelial cells, converts docosapentaenoate (DPA, C22:5(n-3)) to 13R-HDPA, a precursor for 13-series resolvins (RvTs) shown to activate macrophage phagocytosis during bacterial infection. In activated leukocytes, contributes to oxygenation of hydroxyeicosatetraenoates (HETE) to diHETES (5,15-diHETE and 5,11-diHETE). Can also use linoleate (LA, (9Z,12Z)-octadecadienoate, C18:2(n-6)) as substrate and produce hydroxyoctadecadienoates (HODEs) in a regio- and stereospecific manner, being (9R)-HODE ((9R)-hydroxy-(10E,12Z)-octadecadienoate) and (13S)-HODE ((13S)-hydroxy-(9Z,11E)-octadecadienoate) its major products. During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs) 15R-lipoxin A4 that regulates phagocytic microglia.
Protein Name Prostaglandin G/H Synthase 2
Cyclooxygenase-2
Cox-2
Phs Ii
Prostaglandin H2 Synthase 2
Pgh Synthase 2
Pghs-2
Prostaglandin-Endoperoxide Synthase 2
Database Links Reactome: R-HSA-197264
Reactome: R-HSA-2142770
Reactome: R-HSA-2162123
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-9018677
Reactome: R-HSA-9018679
Reactome: R-HSA-9025094
Reactome: R-HSA-9027604
Cellular Localisation Microsome Membrane
Peripheral Membrane Protein
Endoplasmic Reticulum Membrane
Nucleus Inner Membrane
Nucleus Outer Membrane
Detected On The Lumenal Side Of The Endoplasmic Reticulum And Nuclear Envelope
Alternative Antibody Names Anti-Prostaglandin G/H Synthase 2 antibody
Anti-Cyclooxygenase-2 antibody
Anti-Cox-2 antibody
Anti-Phs Ii antibody
Anti-Prostaglandin H2 Synthase 2 antibody
Anti-Pgh Synthase 2 antibody
Anti-Pghs-2 antibody
Anti-Prostaglandin-Endoperoxide Synthase 2 antibody
Anti-PTGS2 antibody
Anti-COX2 antibody

Information sourced from Uniprot.org

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