• Western blot analysis of extracts of various cell lines, using PTBP2 rabbit monoclonal antibody (STJ11102427) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of PTBP2 in paraffin-embedded human cervix cancer tissue using PTBP2 rabbit monoclonal antibody (STJ11102427) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PTBP2 in paraffin-embedded human colon carcinoma tissue using PTBP2 rabbit monoclonal antibody (STJ11102427) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PTBP2 in paraffin-embedded human colon tissue using PTBP2 rabbit monoclonal antibody (STJ11102427) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PTBP2 in paraffin-embedded human thyroid cancer tissue using PTBP2 rabbit monoclonal antibody (STJ11102427) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PTBP2 in paraffin-embedded mouse brain tissue using PTBP2 rabbit monoclonal antibody (STJ11102427) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PTBP2 in paraffin-embedded mouse intestin tissue using PTBP2 rabbit monoclonal antibody (STJ11102427) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PTBP2 in paraffin-embedded mouse liver tissue using PTBP2 rabbit monoclonal antibody (STJ11102427) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PTBP2 in paraffin-embedded mouse testis tissue using PTBP2 rabbit monoclonal antibody (STJ11102427) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PTBP2 in paraffin-embedded rat brain tissue using PTBP2 rabbit monoclonal antibody (STJ11102427) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PTBP2 in paraffin-embedded rat kidney tissue using PTBP2 rabbit monoclonal antibody (STJ11102427) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of U-2 OS cells using PTBP2 rabbit monoclonal antibody (STJ11102427) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PTBP2 antibody (1-100) [S7MR] (STJ11102427)

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STJ11102427

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PTBP2 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTBP2
Gene ID: 58155
Uniprot ID: PTBP2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PTBP2 (Q9UKA9).
Immunogen Sequence: MDGIVTEVAVGVKRGSDELL SGSVLSSPNSNMSSMVVTAN GNDSKKFKGEDKMDGAPSRV LHIRKLPGEVTETEVIALGL PFGKVTNILMLKGKNQAFLE
Tissue Specificity Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform 6 are truncated forms expressed in non-neuronal tissues.
Function RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. In addition to its function in pre-mRNA splicing, plays also a role in the regulation of translation. Isoform 5: Reduced affinity for RNA.
Protein Name Polypyrimidine Tract-Binding Protein 2
Neural Polypyrimidine Tract-Binding Protein
Neurally-Enriched Homolog Of Ptb
Ptb-Like Protein
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Polypyrimidine Tract-Binding Protein 2 antibody
Anti-Neural Polypyrimidine Tract-Binding Protein antibody
Anti-Neurally-Enriched Homolog Of Ptb antibody
Anti-Ptb-Like Protein antibody
Anti-PTBP2 antibody
Anti-NPTB antibody
Anti-PTB antibody
Anti-PTBLP antibody

Information sourced from Uniprot.org

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