| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PSTPIP1 (157-416) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PSTPIP1 |
| Gene ID: |
9051 |
| Uniprot ID: |
PPIP1_HUMAN |
| Immunogen Region: |
157-416 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 157-416 of human PSTPIP1 (NP_003969.2). |
| Immunogen Sequence: |
AFERISANGHQKQVEKSQNK ARQCKDSATEAERVYRQSIA QLEKVRAEWEQEHRTTCEAF QLQEFDRLTILRNALWVHSN QLSMQCVKDDELYEEVRLTL EGCSIDADIDSFIQAKSTGT EPPAPVPYQNYYDREVTPLT SSPGIQPSCGMIKRFSGLLH GSPKTTSLAASAASTETLTP TPERNEGVYTAIAVQEIQGN PASPAQEYRALYDYTAQNPD ELDLSAGDILEVILEGEDG |
| Tissue Specificity | Highly expressed in the peripheral blood leukocytes, granulocytes and monocytes, namely in T-cells and natural killer cells, and in spleen. Weakly expressed in the thymus, small intestine, lung and placenta. |
| Post Translational Modifications | Dephosphorylated on Tyr-345 by PTPN18, this event negatively regulates the association of PSTPIP1 with SH2 domain-containing proteins as tyrosine kinase. Phosphorylation of Tyr-345 is probably required for subsequent phosphorylation at other tyrosine residues. Phosphorylation is induced by activation of the EGFR and PDGFR in a ABL1 dependent manner. The phosphorylation regulates the interaction with WAS and with MEFV. |
| Function | Involved in regulation of the actin cytoskeleton. May regulate WAS actin-bundling activity. Bridges the interaction between ABL1 and PTPN18 leading to ABL1 dephosphorylation. May play a role as a scaffold protein between PTPN12 and WAS and allow PTPN12 to dephosphorylate WAS. Has the potential to physically couple CD2 and CD2AP to WAS. Acts downstream of CD2 and CD2AP to recruit WAS to the T-cell:APC contact site so as to promote the actin polymerization required for synapse induction during T-cell activation. Down-regulates CD2-stimulated adhesion through the coupling of PTPN12 to CD2. Also has a role in innate immunity and the inflammatory response. Recruited to inflammasomes by MEFV. Induces formation of pyroptosomes, large supramolecular structures composed of oligomerized PYCARD dimers which form prior to inflammatory apoptosis. Binding to MEFV allows MEFV to bind to PYCARD and facilitates pyroptosome formation. Regulates endocytosis and cell migration in neutrophils. |
| Protein Name | Proline-Serine-Threonine Phosphatase-Interacting Protein 1Pest Phosphatase-Interacting Protein 1Cd2-Binding Protein 1H-Pip |
| Database Links | Reactome: R-HSA-844456Reactome: R-HSA-9660826 |
| Cellular Localisation | CytoplasmCell MembranePeripheral Membrane ProteinCell ProjectionUropodiumCytoskeletonPerinuclear RegionLamellipodiumCleavage FurrowMainly Cytoplasmic In T-CellsColocalizes In Cluster With Cd2 Near The Cell Surface Membrane In Activated T-CellsIn MonocytesForms A Branched Filamentous Network In The CytoplasmIn Transfected CellsForms Relatively Straight Filaments Radiating Out From The NucleusFilament Formation Requires An Intact Tubulin CytoskeletonIn Migrating NeutrophilsColocalizes With Pip5k1c And Dnm2 To The Trailing Edge Of The Uropod In A Actin-Dependent MannerColocalized With Ptpn12 In The Cytoplasm And The Perinuclear RegionDuring InterphaseColocalizes With F-Actin In The Cortical CytoskeletonLamellipodiaAnd Stress FibersIn Dividing CellsColocalizes With The F-Actin Rich Cytokinetic Cleavage FurrowColocalized With Cd2ap And Was In The Actin Cytoskeleton Within The CytoplasmColocalized With Cd2Cd2ap And Was At The Site Of T-Cell:apc Contact |
| Alternative Antibody Names | Anti-Proline-Serine-Threonine Phosphatase-Interacting Protein 1 antibodyAnti-Pest Phosphatase-Interacting Protein 1 antibodyAnti-Cd2-Binding Protein 1 antibodyAnti-H-Pip antibodyAnti-PSTPIP1 antibodyAnti-CD2BP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
