• Western blot analysis of various lysates, using PSMF1 rabbit polyclonal antibody (STJ27500) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of HepG2 cells using PSMF1 rabbit polyclonal antibody (STJ27500) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PSMF1 antibody (1-271) (STJ27500)

SKU:
STJ27500

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PSMF1 (1-271) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PSMF1
Gene ID: 9491
Uniprot ID: PSMF1_HUMAN
Immunogen Region: 1-271
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human PSMF1 (NP_006805.2).
Immunogen Sequence: MAGLEVLFASAAPAITCRQD ALVCFLHWEVVTHGYFGLGV GDQPGPNDKKSELLPAGWNN NKDLYVLRYEYKDGSRKLLV KAITVESSMILNVLEYGSQQ VADLTLNLDDYIDAEHLGDF HRTYKNSEELRSRIVSGIIT PIHEQWEKANVSSPHREFPP ATAREVDPLRIPPHHPHTSR QPPWCDPLGPFVVGGEDLDP FGPRRGGMIVDPLRSGFPRA LIDPSSGLPNRLPPGAVPP
Function Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.
Protein Name Proteasome Inhibitor Pi31 Subunit
Hpi31
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1234176
Reactome: R-HSA-1236974
Reactome: R-HSA-1236978
Reactome: R-HSA-174084
Reactome: R-HSA-174113
Reactome: R-HSA-174154
Reactome: R-HSA-174178
Reactome: R-HSA-174184
Reactome: R-HSA-180534
Reactome: R-HSA-180585
Reactome: R-HSA-187577
Reactome: R-HSA-195253
Reactome: R-HSA-202424
Reactome: R-HSA-211733
Reactome: R-HSA-2467813
Reactome: R-HSA-2871837
Reactome: R-HSA-349425
Reactome: R-HSA-350562
Reactome: R-HSA-382556
Reactome: R-HSA-450408
Reactome: R-HSA-4608870
Reactome: R-HSA-4641257
Reactome: R-HSA-4641258
Reactome: R-HSA-5358346
Reactome: R-HSA-5362768
Reactome: R-HSA-5607761
Reactome: R-HSA-5607764
Reactome: R-HSA-5610780
Reactome: R-HSA-5610783
Reactome: R-HSA-5610785
Reactome: R-HSA-5632684
Reactome: R-HSA-5658442
Reactome: R-HSA-5668541
Reactome: R-HSA-5676590
Reactome: R-HSA-5678895
Reactome: R-HSA-5687128
Reactome: R-HSA-5689603
Reactome: R-HSA-5689880
Reactome: R-HSA-68867
Reactome: R-HSA-68949
Reactome: R-HSA-69017
Reactome: R-HSA-69481
Reactome: R-HSA-69601
Reactome: R-HSA-75815
Reactome: R-HSA-8852276
Reactome: R-HSA-8854050
Reactome: R-HSA-8939236
Reactome: R-HSA-8939902
Reactome: R-HSA-8941858
Reactome: R-HSA-8948751
Reactome: R-HSA-8951664
Reactome: R-HSA-9010553
Reactome: R-HSA-9020702
Reactome: R-HSA-9604323
Reactome: R-HSA-9755511
Reactome: R-HSA-9762114
Reactome: R-HSA-9824272
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Endoplasmic Reticulum
Alternative Antibody Names Anti-Proteasome Inhibitor Pi31 Subunit antibody
Anti-Hpi31 antibody
Anti-PSMF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance