• Immunofluorescence analysis of HeLa cells using PSMB10 Rabbit polyclonal antibody (STJ27405). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PSMB10 in paraffin-embedded mouse brain using PSMB10 Rabbit polyclonal antibody (STJ27405) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PSMB10 in paraffin-embedded rat brain using PSMB10 Rabbit polyclonal antibody (STJ27405) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from THP-1 cells, using PSMB10 Rabbit polyclonal antibody (STJ27405) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PSMB10 antibody (1-273) (STJ27405)

SKU:
STJ27405

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PSMB10 (1-273) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PSMB10
Gene ID: 5699
Uniprot ID: PSB10_HUMAN
Immunogen Region: 1-273
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human PSMB10 (NP_002792.1).
Immunogen Sequence: MLKPALEPRGGFSFENCQRN ASLERVLPGLKVPHARKTGT TIAGLVFQDGVILGADTRAT NDSVVADKSCEKIHFIAPKI YCCGAGVAADAEMTTRMVAS KMELHALSTGREPRVATVTR ILRQTLFRYQGHVGASLIVG GVDLTGPQLYGVHPHGSYSR LPFTALGSGQDAALAVLEDR FQPNMTLEAAQGLLVEAVTA GILGDLGSGGNVDACVITKT GAKLLRTLSSPTEPVKRSG
Post Translational Modifications Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
Protein Name Proteasome Subunit Beta Type-10
Low Molecular Mass Protein 10
Macropain Subunit Mecl-1
Multicatalytic Endopeptidase Complex Subunit Mecl-1
Proteasome Mecl-1
Proteasome Subunit Beta-2i
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1234176
Reactome: R-HSA-1236974
Reactome: R-HSA-1236978
Reactome: R-HSA-174084
Reactome: R-HSA-174113
Reactome: R-HSA-174154
Reactome: R-HSA-174178
Reactome: R-HSA-174184
Reactome: R-HSA-180534
Reactome: R-HSA-180585
Reactome: R-HSA-187577
Reactome: R-HSA-195253
Reactome: R-HSA-202424
Reactome: R-HSA-211733
Reactome: R-HSA-2467813
Reactome: R-HSA-2871837
Reactome: R-HSA-349425
Reactome: R-HSA-350562
Reactome: R-HSA-382556
Reactome: R-HSA-450408
Reactome: R-HSA-4608870
Reactome: R-HSA-4641257
Reactome: R-HSA-4641258
Reactome: R-HSA-5358346
Reactome: R-HSA-5362768
Reactome: R-HSA-5607761
Reactome: R-HSA-5607764
Reactome: R-HSA-5610780
Reactome: R-HSA-5610783
Reactome: R-HSA-5610785
Reactome: R-HSA-5632684
Reactome: R-HSA-5658442
Reactome: R-HSA-5668541
Reactome: R-HSA-5676590
Reactome: R-HSA-5678895
Reactome: R-HSA-5687128
Reactome: R-HSA-5689603
Reactome: R-HSA-5689880
Reactome: R-HSA-68867
Reactome: R-HSA-68949
Reactome: R-HSA-69017
Reactome: R-HSA-69481
Reactome: R-HSA-69601
Reactome: R-HSA-75815
Reactome: R-HSA-8852276
Reactome: R-HSA-8854050
Reactome: R-HSA-8939236
Reactome: R-HSA-8939902
Reactome: R-HSA-8941858
Reactome: R-HSA-8948751
Reactome: R-HSA-8951664
Reactome: R-HSA-9010553
Reactome: R-HSA-9020702
Reactome: R-HSA-9604323
Reactome: R-HSA-9755511
Reactome: R-HSA-9762114
Reactome: R-HSA-9824272
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Proteasome Subunit Beta Type-10 antibody
Anti-Low Molecular Mass Protein 10 antibody
Anti-Macropain Subunit Mecl-1 antibody
Anti-Multicatalytic Endopeptidase Complex Subunit Mecl-1 antibody
Anti-Proteasome Mecl-1 antibody
Anti-Proteasome Subunit Beta-2i antibody
Anti-PSMB10 antibody
Anti-LMP10 antibody
Anti-MECL1 antibody

Information sourced from Uniprot.org

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