• Immunohistochemistry analysis of paraffin-embedded mouse spleen using PRPF4 Rabbit polyclonal antibody (STJ27846) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using PRPF4 Rabbit polyclonal antibody (STJ27846) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human brain using PRPF4 Rabbit polyclonal antibody (STJ27846) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using PRPF4 Rabbit polyclonal antibody (STJ27846) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using PRPF4 Rabbit polyclonal antibody (STJ27846) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PRPF4 antibody (STJ27846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PRPF4 antibody (1-260) (STJ27846)

SKU:
STJ27846

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRPF4 (1-260) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRPF4
Gene ID: 9128
Uniprot ID: PRP4_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PRPF4 (NP_004688.2).
Immunogen Sequence: MASSRASSTQATKTKAPDDL VAPVVKKPHIYYGSLEEKER ERLAKGESGILGKDGLKAGI EAGNINITSGEVFEIEEHIS ERQAEVLAEFERRKRARQIN VSTDDSEVKACLRALGEPIT LFGEGPAERRERLRNILSVV GTDALKKTKKDDEKSKKSKE EYQQTWYHEGPNSLKVARLW IANYSLPRAMKRLEEARLHK EIPETTRTSQMQELHKSLRS LNNFCSQIGDDRPISYCHF
Function Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).
Protein Name U4/U6 Small Nuclear Ribonucleoprotein Prp4
Prp4 Homolog
Hprp4
U4/U6 Snrnp 60 Kda Protein
Wd Splicing Factor Prp4
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Nucleus Speckle
Alternative Antibody Names Anti-U4/U6 Small Nuclear Ribonucleoprotein Prp4 antibody
Anti-Prp4 Homolog antibody
Anti-Hprp4 antibody
Anti-U4/U6 Snrnp 60 Kda Protein antibody
Anti-Wd Splicing Factor Prp4 antibody
Anti-PRPF4 antibody
Anti-PRP4 antibody

Information sourced from Uniprot.org

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