| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PRPF4 (1-260) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PRPF4 |
| Gene ID: |
9128 |
| Uniprot ID: |
PRP4_HUMAN |
| Immunogen Region: |
1-260 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PRPF4 (NP_004688.2). |
| Immunogen Sequence: |
MASSRASSTQATKTKAPDDL VAPVVKKPHIYYGSLEEKER ERLAKGESGILGKDGLKAGI EAGNINITSGEVFEIEEHIS ERQAEVLAEFERRKRARQIN VSTDDSEVKACLRALGEPIT LFGEGPAERRERLRNILSVV GTDALKKTKKDDEKSKKSKE EYQQTWYHEGPNSLKVARLW IANYSLPRAMKRLEEARLHK EIPETTRTSQMQELHKSLRS LNNFCSQIGDDRPISYCHF |
| Function | Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). |
| Protein Name | U4/U6 Small Nuclear Ribonucleoprotein Prp4Prp4 HomologHprp4U4/U6 Snrnp 60 Kda ProteinWd Splicing Factor Prp4 |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | NucleusNucleus Speckle |
| Alternative Antibody Names | Anti-U4/U6 Small Nuclear Ribonucleoprotein Prp4 antibodyAnti-Prp4 Homolog antibodyAnti-Hprp4 antibodyAnti-U4/U6 Snrnp 60 Kda Protein antibodyAnti-Wd Splicing Factor Prp4 antibodyAnti-PRPF4 antibodyAnti-PRP4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")