| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PRPF3 |
| Gene ID: | 9129 |
| Uniprot ID: | PRPF3_HUMAN |
| Immunogen Region: | 1-280 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PRPF3 (NP_004689.1). |
| Immunogen Sequence: | MALSKRELDELKPWIEKTVK RVLGFSEPTVVTAALNCVGK GMDKKKAADHLKPFLDDSTL RFVDKLFEAVEEGRSSRHSK SSSDRSRKRELKEVFGDDSE ISKESSGVKKRRIPRFEEVE EEPEVIPGPPSESPGMLTKL QIKQMMEAATRQIEERKKQL SFISPPTPQPKTPSSSQPER LPIGNTIQPSQAATFMNDAI EKARKAAELQARIQAQLALK PGLIGNANMVGLANLHAMG |
| Tissue Specificity | Highly expressed in retina, liver, kidney and blood. Detected at lower levels in heart and brain. |
| Post Translational Modifications | Ubiquitinated. Undergoes 'Lys-63'-linked polyubiquitination by PRPF19 and deubiquitination by USP4. 'Lys-63'-linked ubiquitination increases the affinity for PRPF8 and may regulate the assembly of the U4/U6-U5 tri-snRNP complex. |
| Function | Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). |
| Protein Name | U4/U6 Small Nuclear Ribonucleoprotein Prp3Pre-Mrna-Splicing Factor 3Hprp3U4/U6 Snrnp 90 Kda Protein |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | NucleusNucleus Speckle |
| Alternative Antibody Names | Anti-U4/U6 Small Nuclear Ribonucleoprotein Prp3 antibodyAnti-Pre-Mrna-Splicing Factor 3 antibodyAnti-Hprp3 antibodyAnti-U4/U6 Snrnp 90 Kda Protein antibodyAnti-PRPF3 antibodyAnti-HPRP3 antibodyAnti-PRP3 antibody |
Information sourced from Uniprot.org