• Immunofluorescence analysis of U2OS cells using PRPF3 Rabbit polyclonal antibody (STJ27435). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PRPF3 in paraffin-embedded mouse brain using PRPF3 Rabbit polyclonal antibody (STJ27435) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PRPF3 in paraffin-embedded human stomach using PRPF3 Rabbit polyclonal antibody (STJ27435) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PRPF3 in paraffin-embedded rat brain using PRPF3 Rabbit polyclonal antibody (STJ27435) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from THP-1 cells, using PRPF3 Rabbit polyclonal antibody (STJ27435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-PRPF3 antibody (1-280) (STJ27435)

SKU:
STJ27435

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRPF3 (1-280) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRPF3
Gene ID: 9129
Uniprot ID: PRPF3_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PRPF3 (NP_004689.1).
Immunogen Sequence: MALSKRELDELKPWIEKTVK RVLGFSEPTVVTAALNCVGK GMDKKKAADHLKPFLDDSTL RFVDKLFEAVEEGRSSRHSK SSSDRSRKRELKEVFGDDSE ISKESSGVKKRRIPRFEEVE EEPEVIPGPPSESPGMLTKL QIKQMMEAATRQIEERKKQL SFISPPTPQPKTPSSSQPER LPIGNTIQPSQAATFMNDAI EKARKAAELQARIQAQLALK PGLIGNANMVGLANLHAMG
Tissue Specificity Highly expressed in retina, liver, kidney and blood. Detected at lower levels in heart and brain.
Post Translational Modifications Ubiquitinated. Undergoes 'Lys-63'-linked polyubiquitination by PRPF19 and deubiquitination by USP4. 'Lys-63'-linked ubiquitination increases the affinity for PRPF8 and may regulate the assembly of the U4/U6-U5 tri-snRNP complex.
Function Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).
Protein Name U4/U6 Small Nuclear Ribonucleoprotein Prp3
Pre-Mrna-Splicing Factor 3
Hprp3
U4/U6 Snrnp 90 Kda Protein
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Nucleus Speckle
Alternative Antibody Names Anti-U4/U6 Small Nuclear Ribonucleoprotein Prp3 antibody
Anti-Pre-Mrna-Splicing Factor 3 antibody
Anti-Hprp3 antibody
Anti-U4/U6 Snrnp 90 Kda Protein antibody
Anti-PRPF3 antibody
Anti-HPRP3 antibody
Anti-PRP3 antibody

Information sourced from Uniprot.org

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