• Western blot analysis of 5ng Mouse Pro-MMP9 protein, using Pro-MMP9 Rabbit monoclonal antibody (STJ11105257) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 3s.

Anti-Pro-MMP9 antibody (20-107) [S5257RM] (STJ11105257)

SKU:
STJ11105257

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Pro-MMP9 (20-107) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5257RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Mmp9
Gene ID: 17395
Uniprot ID: MMP9_MOUSE
Immunogen Region: 20-107
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-107 of mouse MMP9 (NP_038627.1).
Immunogen Sequence: APYQRQPTFVVFPKDLKTSN LTDTQLAEAYLYRYGYTRAA QMMGEKQSLRPALLMLQKQL SLPQTGELDSQTLKAIRTPR CGVPDVGR
Post Translational Modifications N- and O-glycosylated.
Function Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Protein Name Matrix Metalloproteinase-9
Mmp-9
92 Kda Gelatinase
92 Kda Type Iv Collagenase
Gelatinase B
Gelb
Database Links Reactome: R-MMU-1433557
Reactome: -MMU-1442490
Reactome: -MMU-1474228
Reactome: -MMU-1592389
Reactome: -MMU-2022090
Reactome: -MMU-3928665
Reactome: -MMU-6798695
Reactome: -MMU-9009391
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Matrix Metalloproteinase-9 antibody
Anti-Mmp-9 antibody
Anti-92 Kda Gelatinase antibody
Anti-92 Kda Type Iv Collagenase antibody
Anti-Gelatinase B antibody
Anti-Gelb antibody
Anti-Mmp9 antibody
Anti-Clg4b antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance