![Western blot analysis of lysates from wild type (WT) and PRMT3 knockout (KO) 293T cells, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94075/375453/STJ115035_1__47849.1713142217.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PRMT3 knockout (KO) 293T cells, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western blot analysis of lysates from mouse brain, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94075/375454/STJ115035_2__22315.1713142218.jpg?c=1 "Western blot analysis of lysates from mouse brain, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western blot analysis of lysates from wild type (WT) and PRMT3 knockout (KO) 293T cells, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/94075/375453/STJ115035_1__47849.1713142217.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PRMT3 knockout (KO) 293T cells, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PRMT3 |
| Gene ID: | 10196 |
| Uniprot ID: | ANM3_HUMAN |
| Immunogen Region: | 1-220 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PRMT3 (NP_005779.1). |
| Immunogen Sequence: | MCSLASGATGGRGAVENEED LPELSDSGDEAAWEDEDDAD LPHGKQQTPCLFCNRLFTSA EETFSHCKSEHQFNIDSMVH KHGLEFYGYIKLINFIRLKN PTVEYMNSIYNPVPWEKEEY LKPVLEDDLLLQFDVEDLYE PVSVPFSYPNGLSENTSVVE KLKHMEARALSAEAALARAR EDLQKMKQFAQDFVMHTDVR TCSSSTSVIADLQEDEDGVY |
| Function | Protein-arginine N-methyltransferase that catalyzes both the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, and therefore falls into the group of type I methyltransferases (Probable). May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity. |
| Protein Name | Protein Arginine N-Methyltransferase 3Heterogeneous Nuclear Ribonucleoprotein Methyltransferase-Like Protein 3 |
| Database Links | Reactome: R-HSA-3214858Reactome: R-HSA-8876725 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Protein Arginine N-Methyltransferase 3 antibodyAnti-Heterogeneous Nuclear Ribonucleoprotein Methyltransferase-Like Protein 3 antibodyAnti-PRMT3 antibodyAnti-HRMT1L3 antibody |
Information sourced from Uniprot.org