• Western blot analysis of lysates from wild type (WT) and PRMT3 knockout (KO) 293T cells, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of lysates from mouse brain, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PRMT3 antibody (1-220) (STJ115035)

SKU:
STJ115035

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRMT3 (1-220) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRMT3
Gene ID: 10196
Uniprot ID: ANM3_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PRMT3 (NP_005779.1).
Immunogen Sequence: MCSLASGATGGRGAVENEED LPELSDSGDEAAWEDEDDAD LPHGKQQTPCLFCNRLFTSA EETFSHCKSEHQFNIDSMVH KHGLEFYGYIKLINFIRLKN PTVEYMNSIYNPVPWEKEEY LKPVLEDDLLLQFDVEDLYE PVSVPFSYPNGLSENTSVVE KLKHMEARALSAEAALARAR EDLQKMKQFAQDFVMHTDVR TCSSSTSVIADLQEDEDGVY
Function Protein-arginine N-methyltransferase that catalyzes both the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, and therefore falls into the group of type I methyltransferases (Probable). May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity.
Protein Name Protein Arginine N-Methyltransferase 3
Heterogeneous Nuclear Ribonucleoprotein Methyltransferase-Like Protein 3
Database Links Reactome: R-HSA-3214858
Reactome: R-HSA-8876725
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Protein Arginine N-Methyltransferase 3 antibody
Anti-Heterogeneous Nuclear Ribonucleoprotein Methyltransferase-Like Protein 3 antibody
Anti-PRMT3 antibody
Anti-HRMT1L3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance