| Host: |
Mouse |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Protein arginine N-methyltransferase 1 is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
4E9-H9-H12 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2b |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PRMT1 |
| Gene ID: |
3276 |
| Uniprot ID: |
ANM1_HUMAN |
| Specificity: |
This antibody detects endogenous levels of PRMT1 and does not cross-react with related proteins. |
| Immunogen: |
Purified recombinant human PRMT1 protein fragments expressed in E.coli. |
| Post Translational Modifications | Polyubiquitinated at Lys-145 by the SCF(FBXL17) complex, leading to its subsequent degradation. Ubiquitination is regulated by acetylation at Lys-228 and Lys-233. Polyubiquitinated by E3 ubiquitin-protein ligase TRIM48, leading to suppression of MAP3K5/ASK1 methylation and subsequent MAP3K5 activation. Acetylation at Lys-228 and Lys-233 regulates ubiquitination by the SCF(FBXL17) complex. Acetylated at Lys-233 by p300/EP300. Deacetylated at Lys-228 and Lys-233 by SIRT1. |
| Function | Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, FMR1, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4, SERBP1, RBM15, FOXO1, CHTOP and MAP3K5/ASK1. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity. Methylates MAP3K5/ASK1 at 'Arg-78' and 'Arg-80' which promotes association of MAP3K5 with thioredoxin and negatively regulates MAP3K5 association with TRAF2, inhibiting MAP3K5 stimulation and MAP3K5-induced activation of JNK. Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner. Plays a role in regulating alternative splicing in the heart. |
| Protein Name | Protein Arginine N-Methyltransferase 1Histone-Arginine N-Methyltransferase Prmt1Interferon Receptor 1-Bound Protein 4 |
| Database Links | Reactome: R-HSA-3214858Reactome: R-HSA-6804114Reactome: R-HSA-8936459Reactome: R-HSA-9009391Reactome: R-HSA-9018519Reactome: R-HSA-9694631 |
| Cellular Localisation | NucleusNucleoplasmCytoplasmCytosolMostly Found In The CytoplasmColocalizes With Chtop Within The NucleusLow Levels Detected Also In The Chromatin Fraction |
| Alternative Antibody Names | Anti-Protein Arginine N-Methyltransferase 1 antibodyAnti-Histone-Arginine N-Methyltransferase Prmt1 antibodyAnti-Interferon Receptor 1-Bound Protein 4 antibodyAnti-PRMT1 antibodyAnti-HMT2 antibodyAnti-HRMT1L2 antibodyAnti-IR1B4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
