| Host: |
Rabbit |
| Applications: |
WB/IHC-P |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-PRKRA (260-313 aa) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
R05-4C4 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS pH7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol. |
| Purification: |
Affinity Purified |
| Concentration: |
- |
| Dilution Range: |
WB 1:500-1:1000IHC 1:50-1:100 |
| Storage Instruction: |
Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: |
PRKRA |
| Gene ID: |
8575 |
| Uniprot ID: |
PRKRA_HUMAN |
| Immunogen Region: |
260-313 aa |
| Immunogen: |
A synthesized peptide derived from human PRKRA |
| Post Translational Modifications | Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress. |
| Function | Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner. |
| Protein Name | Interferon-Inducible Double-Stranded Rna-Dependent Protein Kinase Activator APkr-Associated Protein XPkr-Associating Protein XProtein Activator Of The Interferon-Induced Protein KinaseProtein Kinase - Interferon-Inducible Double-Stranded Rna-Dependent Activator |
| Database Links | Reactome: R-HSA-203927Reactome: R-HSA-426486Reactome: R-HSA-9833482 |
| Cellular Localisation | CytoplasmPerinuclear Region |
| Alternative Antibody Names | Anti-Interferon-Inducible Double-Stranded Rna-Dependent Protein Kinase Activator A antibodyAnti-Pkr-Associated Protein X antibodyAnti-Pkr-Associating Protein X antibodyAnti-Protein Activator Of The Interferon-Induced Protein Kinase antibodyAnti-Protein Kinase - Interferon-Inducible Double-Stranded Rna-Dependent Activator antibodyAnti-PRKRA antibodyAnti-PACT antibodyAnti-RAX antibodyAnti-HSD-14 antibodyAnti-HSD14 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
